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Human TACC3 Antibody

Catalog #: AF5720
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5720
AF5720-SP
Detection of Human TACC3 by Western Blot.
2 Images
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TACC3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TACC3 in Western blots.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TACC3 antibody by Western Blot. View Larger

Detection of Human TACC3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TACC3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human TACC3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TACC3 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TACC3 at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TACC3

Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92‑140 kDa member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids (aa) in length and contains one poly-Serine region (aa 155‑160) and a TACC coiled-coil domain (aa 637‑837). There is one potential splice variant that shows a Lys substitution for aa 102‑462. Over amino acids 689‑838, human TACC3 shares 79% aa identity with mouse TACC3.

Long Name
Transforming Acidic Coiled-coil-containing Protein 3
Entrez Gene IDs
10460 (Human); 21335 (Mouse); 360962 (Rat)
Alternate Names
ERIC-1; ERIC1ERIC-1; MGC117382; MGC133242; TACC3; transforming acidic coiled-coil-containing protein 3; transforming, acidic coiled-coil containing protein 3

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Citations for Human TACC3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.
    Authors: Ana G, Bernardo O, Marco N et al.
    Curr Biol.
  2. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
    Authors: So C, Seres K. B, et al.
    Science
  3. Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes
    Authors: WS Yuen, QH Zhang, A Bourdais, D Adhikari, G Halet, J Carroll
    Open Biology, 2023-03-08;13(3):220326.
  4. Targeting FGFRs by pemigatinib induces G1 phase cell cycle arrest, cellular stress and upregulation of tumor suppressor microRNAs
    Authors: Pace, A;Scirocchi, F;Napoletano, C;Zizzari, IG;Po, A;Megiorni, F;Asquino, A;Pontecorvi, P;Rahimi, H;Marchese, C;Ferretti, E;Nuti, M;Rughetti, A;
    Journal of translational medicine
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization
    Authors: Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
    Journal of Cell Science
  6. FGFR3-TACC3 fusion proteins act as naturally occurring drivers of tumor resistance by functionally substituting for EGFR/ERK signaling
    Authors: C Daly, C Castanaro, W Zhang, Q Zhang, Y Wei, M Ni et al.
    Oncogene

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