Human Tenascin X Antibody

Catalog #: MAB6999 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6999
MAB6999-SP

Detection of Human Tenascin X by Western Blot.

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Human Tenascin X Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tenascin XB2 in ELISAs and Western blots. Also detects Tenascin X in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tenascin R is observed.

Source

Monoclonal Mouse IgG_2A Clone # 766842

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Tenascin XB2
Met1-Tyr673
Accession # AAH33740

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tenascin X by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western

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Detection of Human Tenascin X by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Tenascin X at approximately 376 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tenascin X

Tenascin XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene.

Entrez Gene IDs

7148 (Human); 81877 (Mouse); 415089 (Rat)

Alternate Names

Hexabrachion-like protein; HXBL; Tenascin X; tenascin XB; tenascin XB2; tenascin-X; TNX; TNXB; TNXB1growth-inhibiting protein 45; TNXB2tenascin XB1; TNXBS; TNXBSTNXTENX; XB; XBS; XB-S; XBTN-X