Human Testican 2/SPOCK2 Biotinylated Antibody

Catalog #: BAF2328 Datasheet / COA / SDS

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BAF2328

Detection of Rat Testican 2/SPOCK2 by Western Blot.

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Human Testican 2/SPOCK2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Testican 2/SPOCK2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Testican 1 and rhTestican 3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Testican 2/SPOCK2
Glu23-Trp424
Accession # Q92563

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat Testican 2/SPOCK2 by Western Blot. Western blot shows lysates of rat lung tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Testican 2/SPOCK2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2328) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Testican 2/SPOCK2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 2/SPOCK2

Testican 2 (also SPOCK2) is a 60-140 kDa, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402 amino acids (aa) in length (aa 23-424). It contains a unique 66 aa N terminus, followed by a Kazal-like follistatin domain (aa 89-192), a collagen-binding domain (aa 210-219), a Ca⁺⁺ binding region, one thyroglobulin-like segment (aa 310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125 kDa. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of aa 64-66, while a third contains a 14 aa substitution for aa 64-242. Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2.

Entrez Gene IDs

9806 (Human)

Alternate Names

FLJ97039; KIAA0275; KIAA0275CWCV, and Kazal-like domains proteoglycan 2; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2; SPOCK2; Testican 2