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Human Thrombopoietin R/Tpo R APC-conjugated Antibody

Catalog #: FAB1016A
1 Citation Datasheet / COA / SDS
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FAB1016A
Detection of Thrombopoietin R/Tpo R in TF‑1 Human Cell Line by Flow Cytometry.
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Human Thrombopoietin R/Tpo R APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Thrombopoietin R/Tpo R in Western blots. In Western blots, no cross-reactivity with recombinant mouse Tpo R or recombinant human Epo R is observed.
Source
Monoclonal Mouse IgG2A Clone # 167639
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombopoietin R/Tpo R
Ser25-Tyr423
Accession # P40238
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Thrombopoietin R/Tpo R antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Thrombopoietin R/Tpo R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Thrombopoietin R/Tpo R APC-conjugated Monoclonal Antibody (Catalog # FAB1016A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Thrombopoietin R/Tpo R

Thrombopoietin R, also known as Tpo R, is a type I membrane protein belonging to the hematopoietin/cytokine receptor superfamily. Tpo R is expressed by hematopoietic progenitor cells, megakaryocytes and platelets and is involved in the regulation of megakaryocytopoiesis and thrombopoiesis.

Long Name
Thrombopoietin Receptor
Entrez Gene IDs
4352 (Human); 17480 (Mouse)
Alternate Names
CD110 antigen; CD110; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV

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Citation for Human Thrombopoietin R/Tpo R APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Aerobic exercise in humans mobilizes HSC in an intensity dependent manner
    Authors: Gianni Parise
    J. Appl. Physiol., 2016-11-23;0(0):jap.00696.201.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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