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Human Tie-1 Antibody

Catalog #: AF619
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF619
AF619-SP
Detection of Tie‑1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tie-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Tie-1 is observed and less than 1% cross-reactivity with recombinant human Tie‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie-1
Ala22-Gln760
Accession # P35590
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Tie‑1 Fc Chimera (Catalog # 619-TI)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Tie‑1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Tie‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry Tie‑1 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Tie‑1 in Human Placenta. Tie-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-1

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159
  2. Sato, T.N. et al. (1995) Nature 376:70
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Entrez Gene IDs
7075 (Human); 21846 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1

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Citations for Human Tie-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
    Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
    Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
    Species: Mouse
    Sample Types: Protein Lysates
    Applications: Western Blot
  2. Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
    Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
    PloS Pathogens, 2022-01-31;18(1):e1010280.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Sulfated glycans engage the Ang-Tie pathway to regulate vascular development
    Authors: ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils
    Nat Chem Biol, 2020-10-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  4. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
    Authors: VM Leppänen, P Saharinen, K Alitalo
    Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
    Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
    Circ. Res, 2017-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015-01-30;6(0):5962.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  7. Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2.
    Authors: Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP
    J. Biol. Chem., 2007-08-29;282(42):30509-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells.
    Authors: Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK
    Cardiovasc. Res., 2006-08-07;72(3):394-402.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003-07-01;112(1):126-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2.
    Authors: Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP
    J. Biol. Chem., 2000-12-15;275(50):39741-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Human Tie-1 Antibody
By Mohammad Daud Khan on 10/17/2018
Application: IHC Sample Tested: Aortic smooth muscle cells Species: Human
Immunohistochemistry Human Tie-1 Antibody AF619