Human TIMP Luminex® Performance Assay 3-plex Fixed Panel
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Human TIMP Luminex® Performance Assay 3-plex Fixed Panel Summary
| Assay Type | bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and antibody-coated beads |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. |
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.
- Simultaneously profile up to 50 analytes of your choice in one sample
- Choose from a wide menu of analytes, including many unique analytes
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-4 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
Please allow additional time for international orders.
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Standard/Sample Diluent
- Wash Buffer
- Streptavidin-PE
- Foil Plate Sealers (4)
- Mixing Bottle
- Certificate of Analysis**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Precision
|
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays. |
||
| n=40 (20x2 levels) | n=30 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| TIMP-1 | 7.0 | 11.2 |
| TIMP-2 | 4.3 | 9.9 |
| TIMP-4 | 5.8 | 10.8 |
Accuracy
|
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
||
| Linearity | Spiked Recovery |
|
|---|---|---|
| Analyte | Average % | Average % |
| TIMP-1 | 111 | NA |
| TIMP-2 | 100 | 84 |
| TIMP-4 | 88 | 94 |
| NA = Not applicable | ||
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Scientific Data
View Larger
Human TIMP Performance Luminex Panel Serum Sample Values Serum samples from 24 apparently healthy individuals were run on the Human TIMP Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.
Background: TIMP Assay Kits
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
Product Datasheets
Citations for Human TIMP Luminex® Performance Assay 3-plex Fixed Panel
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Paradoxical Changes: EMMPRIN Tissue and Plasma Levels in Marfan Syndrome-Related Thoracic Aortic Aneurysms
Authors: Alexander, KC;Anderson, CW;Agala, CB;Tasoudis, P;Collins, EN;Ding, Y;Blackwell, JW;Willcox, DE;Farivar, BS;Kibbe, MR;Ikonomidis, JS;Akerman, AW;
Journal of clinical medicine
Species: Human hepegivirus
Sample Types: Tissue Homogenates, Plasma
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Anethole induces anti-oral cancer activity by triggering apoptosis, autophagy and oxidative stress and by modulation of multiple signaling pathways
Authors: C Contant, M Rouabhia, L Loubaki, F Chandad, A Semlali
Scientific Reports, 2021-06-22;11(1):13087.
Species: Human
Sample Types: Cell Culture Supernates
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Markers of tissue remodeling and inflammation in the white and brown adipose tissues of a model hibernator
Authors: SM Logan, KB Storey
Cellular Signalling, 2021-03-09;82(0):109975.
Species: Squirrel
Sample Types: Tissue Homogenates
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Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children
Authors: VVS Albuquerqu, NP Kumar, KF Fukutani, B Vasconcelo, MB Arriaga, PS Silveira-M, S Babu, BB Andrade
Cytokine, 2019-07-09;123(0):154773.
Species: Human
Sample Types: Plasma
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Adipose-derived stromal cell secretome reduces TNF?-induced hypertrophy and catabolic markers in primary human articular chondrocytes
Authors: S Niada, C Giannasi, M Gomarasca, D Stanco, S Casati, AT Brini
Stem Cell Res, 2019-05-15;38(0):101463.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of aqueous suppressants and prostaglandin analogues on early wound healing after glaucoma implant surgery
Authors: KI Jung, JE Woo, CK Park
Sci Rep, 2019-03-27;9(1):5251.
Species: Rabbit
Sample Types: Aqueous Humor
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Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
J Immunol, 2015-08-12;195(6):2763-73.
Species: Human
Sample Types: Plasma
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Overexpression of MMP-9 and its inhibitors in blood mononuclear cells after myocardial infarction--is it associated with depressive symptomatology?
Authors: Jonsson S, Lundberg A, Jonasson L
PLoS ONE, 2014-08-25;9(8):e105572.
Species: Human
Sample Types: Cell Culture Supernates
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