Human TLR1 Biotinylated Antibody

Catalog #: BAF1484
2 Citations Datasheet / COA / SDS

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BAF1484

All TLR1 Antibodies

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Citations (2)

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Human TLR1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) TLR1 and rmTLR6 is observed and less than 1% cross-reactivity with recombinant human (rh) TLR3 and rhTLR4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR1
Ser22-Asn578 (Asn228Ser)
Accession # Q15399

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TLR1 Fc Chimera (Catalog # 1484-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (3, 4). Within the ECD, human TLR1 shares 63% aa sequence identity with human TLR6 and 20%‑43% aa sequence identitity with human TLR2, -3, -4, -5, -7, -8, -9, and -10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

References

Miyake, K. (2007) Semin. Immunol. 19:3.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
Matsushima, N. et al. (2007) BMC Genomics 8:124.
Ochoa, M.-T. et al. (2003) Immunology 108:10.
Takeuchi, O. et al. (2002) J. Immunol. 169:10.
Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
Nakao, Y. et al. (2005) J. Immunol. 174:1566.
Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Long Name

Toll-like Receptor 1

Entrez Gene IDs

7096 (Human); 21897 (Mouse); 305354 (Rat)

Alternate Names

CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; TLR1; Toll/interleukin-1 receptor-like protein; toll-like receptor 1

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human TLR1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation
Authors: C Mayslich, PA Grange, M Castela, AG Marcelin, V Calvez, N Dupin
International Journal of Molecular Sciences, 2022-05-03;23(9):.
Species: Human
Sample Types: Protein
Applications: Western Blot
Susceptibility to tuberculosis is associated with TLR1 polymorphisms resulting in a lack of TLR1 cell surface expression.
Authors: Uciechowski P, Imhoff H, Lange C, Meyer CG, Browne EN, Kirsten DK, Schroder AK, Schaaf B, Al-Lahham A, Reinert RR, Reiling N, Haase H, Hatzmann A, Fleischer D, Heussen N, Kleines M, Rink L
J. Leukoc. Biol., 2011-06-03;90(2):377-88.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot