Human TLR3 Antibody

Catalog #: AF1487
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1487
AF1487-SP

All TLR3 Antibodies

Product Details

Citations (9)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR3 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR3
Lys27-Ser711
Accession # O15455

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TLR3 (Catalog # 1487-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8 - 11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11 - 13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13 - 15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

References

Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
Choe, J. et al. (2005) Science 309:581.
Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
Alexopoulou, L. et al. (2001) Nature 413:732.
Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
Rudd, B.D. et al. (2005) J. Virol. 79:3350.
Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
Wang, T. et al. (2004) Nat. Med. 10:1366.

Long Name

Toll-like Receptor 3

Entrez Gene IDs

7098 (Human); 142980 (Mouse); 364594 (Rat)

Alternate Names

CD283 antigen; CD283; TLR3; toll-like receptor 3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TLR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Increased expression of toll-like receptor 3, an anti-viral signaling molecule, and related genes in Alzheimer's disease brains.
Authors: Walker D, Tang T, Lue L
Exp Neurol, 2018-08-01;309(0):91-106.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
J. Biol. Chem., 2017-07-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5
PLoS ONE, 2016-08-09;11(8):e0160875.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Proteolytic processing regulates Toll-like receptor 3 stability and endosomal localization.
Authors: Qi, Rongsu, Singh, Divyendu, Kao, C Cheng
J Biol Chem, 2012-08-03;287(39):32617-29.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot
LL37 and cationic peptides enhance TLR3 signaling by viral double-stranded RNAs.
Authors: Lai Y, Adhikarakunnathu S, Bhardwaj K
PLoS ONE, 2011-10-21;6(10):e26632.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Viral double-strand RNA-binding proteins can enhance innate immune signaling by toll-like Receptor 3.
Authors: Lai Y, Yi G, Chen A
PLoS ONE, 2011-10-10;6(10):e25837.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Herpes simplex virus encephalitis in a patient with complete TLR3 deficiency: TLR3 is otherwise redundant in protective immunity.
Authors: Guo Y, Audry M, Ciancanelli M, Alsina L, Azevedo J, Herman M, Anguiano E, Sancho-Shimizu V, Lorenzo L, Pauwels E, Philippe PB, Perez de Diego R, Cardon A, Vogt G, Picard C, Andrianirina ZZ, Rozenberg F, Lebon P, Plancoulaine S, Tardieu M, ValÃƒÂ©rie Doireau E, Jouanguy D, Chaussabel F, Geissmann L, Abel JL, Casanova SY, Zhang
J. Exp. Med., 2011-09-12;208(10):2083-98.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
Oxidative stress enhances toll-like receptor 3 response to double-stranded RNA in airway epithelial cells.
Authors: Koarai A, Sugiura H, Yanagisawa S
Am. J. Respir. Cell Mol. Biol., 2009-07-13;42(6):651-60.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Activation of Toll-like receptor 3 augments myofibroblast differentiation.
Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
Am. J. Respir. Cell Mol. Biol., 2008-11-06;40(6):654-62.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot