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Human TNF RI/TNFRSF1A PE-conjugated Antibody

Catalog #: FAB225P
17 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB225P
Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RI/TNFRSF1A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 16803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TNF RI/TNFRSF1A antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A PE-conjugated Monoclonal Antibody (Catalog # FAB225P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. The Influence of Severity and Disease Duration on TNF Receptors' Redistribution in Asthma and Rheumatoid Arthritis
    Authors: A Alshevskay, J Lopatnikov, J Zhukova, O Chumasova, N Shkaruba, A Sizikov, I Evsegneeva, D Demina, V Nepomniash, A Karaulov, S Sennikov
    Cells, 2022-12-20;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cytoplasmic FLIP(S) and nuclear FLIP(L) mediate resistance of castrate-resistant prostate cancer to apoptosis induced by IAP antagonists
    Authors: C McCann, N Crawford, J Majkut, C Holohan, CWD Armstrong, PJ Maxwell, CW Ong, MJ LaBonte, SS McDade, DJ Waugh, DB Longley
    Cell Death Dis, 2018-10-22;9(11):1081.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking
    Authors: A Fauster, M Rebsamen, KL Willmann, A César-Razq, E Girardi, JW Bigenzahn, F Schischlik, S Scorzoni, M Bruckner, J Konecka, K Hörmann, LX Heinz, K Boztug, G Superti-Fu
    Cell Death Differ., 2018-09-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tumour necrosis factor receptor I blockade shows that TNF-dependent and TNF-independent mechanisms synergise in TNF receptor associated periodic syndrome.
    Authors: Fairclough L, Stoop A, Negm O, Radford P, Tighe P, Todd I
    Eur J Immunol, 2015-08-17;45(10):2937-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. TNFR2 and IL-12 coactivation enables slanDCs to support NK-cell function via membrane-bound TNF-alpha.
    Authors: Tufa D, Chatterjee D, Low H, Schmidt R, Jacobs R
    Eur J Immunol, 2014-10-29;44(12):3717-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TNFR2 increases the sensitivity of ligand-induced activation of the p38 MAPK and NF-kappaB pathways and signals TRAF2 protein degradation in macrophages.
    Authors: Ruspi G, Schmidt E, McCann F, Feldmann M, Williams R, Stoop A, Dean J
    Cell Signal, 2013-12-27;26(4):683-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection.
    Authors: Cosgrove C, Ussher J, Rauch A, Gartner K, Kurioka A, Huhn M, Adelmann K, Kang Y, Fergusson J, Simmonds P, Goulder P, Hansen T, Fox J, Gunthard H, Khanna N, Powrie F, Steel A, Gazzard B, Phillips R, Frater J, Uhlig H, Klenerman P
    Blood, 2012-12-18;121(6):951-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Secretory lysosome targeting and induced secretion of human soluble TNF-alpha receptor in murine hematopoietic cells in vivo as a principle for immunoregulation in inflammation and malignancy.
    Authors: Johansson AC, Nandakumar KS, Persson AM, Olsson I, Hansson M
    Exp. Hematol., 2009-05-30;37(8):969-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007-10-01;179(7):4840-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007-09-15;179(6):4239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Human cytomegalovirus blocks tumor necrosis factor alpha- and interleukin-1beta-mediated NF-kappaB signaling.
    Authors: Montag C, Wagner J, Gruska I, Hagemeier C
    J. Virol., 2006-09-27;80(23):11686-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Modeling of tumor necrosis factor receptor superfamily 1A mutants associated with tumor necrosis factor receptor-associated periodic syndrome indicates misfolding consistent with abnormal function.
    Authors: Rebelo SL, Bainbridge SE, Amel-Kashipaz MR, Radford PM, Powell RJ, Todd I, Tighe PJ
    Arthritis Rheum., 2006-08-01;54(8):2674-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Neutrophils from patients with TNFRSF1A mutations display resistance to tumor necrosis factor-induced apoptosis: pathogenetic and clinical implications.
    Authors: D'Osualdo A
    Arthritis Rheum., 2006-03-01;54(3):998-1008.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-alpha and its possible role in hemophagocytic syndrome.
    Authors: Saito K, Hirokawa M, Inaba K, Fukaya H, Kawabata Y, Komatsuda A, Yamashita J, Sawada K
    Blood, 2005-10-18;107(4):1366-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Codevelopment of dendritic cells along with erythroid differentiation from human CD34(+) cells by tumor necrosis factor-alpha.
    Authors: Fukaya H, Xiao W, Inaba K, Suzuki Y, Hirokawa M, Kawabata Y, Komatsuda A, Endo T, Kishimoto H, Takada G, Sawada K
    Exp. Hematol., 2004-05-01;32(5):450-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Tumor necrosis factor-alpha inhibits generation of glycophorin A+ cells by CD34+ cells.
    Authors: Xiao W, Koizumi K, Nishio M, Endo T, Osawa M, Fujimoto K, Sato I, Sakai T, Koike T, Sawada K
    Exp. Hematol., 2002-11-01;30(11):1238-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Inhibition of programmed cell death impairs in vitro vascular-like structure formation and reduces in vivo angiogenesis.
    Authors: Segura I, 2019, Serrano A, De Buitrago GG, Gonzalez MA, Abad JL, Claveria C, Gomez L, Bernad A, Martinez-A C, Riese HH
    7096, 2002-06-01;16(8):833-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human TNF RI/TNFRSF1A PE-conjugated Antibody

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Human TNF RI/TNFRSF1A PE-conjugated Antibody
By Christian Ostalecki on 06/07/2018
Application: ICC/IF Sample Tested: Pyoderma gangrenosum,Skin tissue Species: Human
Immunocytochemistry/Immunofluorescence Human TNF RI/TNFRSF1A PE-conjugated Antibody FAB225P
Human TNF RI/TNFRSF1A PE-conjugated Antibody
By Barbara Aguilar on 10/20/2017
Application: Flow Sample Tested: Tranfected Cell Line Species: Human