Human TPT1/TCTP Antibody

Catalog # Availability Size / Price Qty
AF4648
AF4648-SP
Detection of Human TPT1/TCTP by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human TPT1/TCTP Antibody Summary

Species Reactivity
Human
Specificity
Detects human TPT1/TCTP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TPT1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TPT1/TCTP antibody by Western Blot. View Larger

Detection of Human TPT1/TCTP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TPT1/TCTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TPT1/TCTP at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TPT1/TCTP

TPT-1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 22-23 kDa member of the TCTP family of calcium-binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator (Oct4), calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT-1 is 172 amino acids (aa) in length. It contains little in the way of structural motifs. There are multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99-172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1-172, human TPT-1 is 96% aa identical to mouse TPT-1.

Long Name
Translationally-controlled Tumor Protein 1
Entrez Gene IDs
7178 (Human); 22070 (Mouse); 116646 (Rat)
Alternate Names
FLJ27337; Fortilin; Histamine-releasing factor; HRF; p02; p23; TCTP; TCTPHRFfortilin; TPT1; translationally-controlled tumor protein; tumor protein, translationally-controlled 1

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TPT1/TCTP Antibody

There are currently no reviews for this product. Be the first to review Human TPT1/TCTP Antibody and earn rewards!

Have you used Human TPT1/TCTP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review