Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (F0116).
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TRA‑1‑60 in BG01V Human Stem Cells. TRA-1-60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines. TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Detection of TRA‑1‑60(R) in iPSC cells by Flow Cytometry. iPSC cells were stained with Mouse Anti-Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody Mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Product Datasheets
Citations for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
Stem Cell Research, 2021-10-22;57(0):102583.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
Authors: DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah
Scientific Reports, 2021-04-19;11(1):8523.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines
Authors: SJ Walker, AL Wagoner, D Leavitt, DL Mack
Heliyon, 2021-04-03;7(4):e06617.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies
Authors: Y Nagai, H Nakao, A Kojima, Y Komatsubar, Y Ohta, N Kawasaki, N Kawasaki, H Toyoda, T Kawasaki
Biomolecules, 2021-03-29;11(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptional signatures of participant-derived neural progenitor cells and neurons implicate altered Wnt signaling in Phelan-McDermid syndrome and autism
Authors: MS Breen, A Browne, GE Hoffman, S Stathopoul, K Brennand, JD Buxbaum, E Drapeau
Mol Autism, 2020-06-19;11(1):53.
Species: Human
Sample Types: Whole Cells
Applications: Labeling -
Generation of three induced pluripotent stem cell lines from postmortem tissue derived following sudden death of a young patient with STXBP1 mutation
Authors: T Yamamoto, M Otsu, T Okumura, Y Horie, Y Ueno, H Taniguchi, M Ohtaka, M Nakanishi, Y Abe, T Murase, T Umehara, K Ikematsu
Stem Cell Res, 2019-06-18;39(0):101485.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, IHC -
Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
Stem Cell Res, 2017-03-07;20(0):105-114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
BMC Vet Res, 2012-08-31;8(0):150.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
Authors: Kim SY, Kim MJ, Jung H
Stem Cells Dev., 2011-08-29;21(8):1272-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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The cells were stained with 1 µg of TRA-1-60 antibody in 100 µl FACS buffer at 4C for 30 min. After washing, the cells were incubated with anti-IgM-AF488 antibody at 4C for 20 min.
