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Human TRA-1-85/CD147 Biotinylated Antibody

Catalog #: BAM3195 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAM3195
TRA‑1‑85/CD147 in THP‑1 Human Cell Line.
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Human TRA-1-85/CD147 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑85.
Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot

Williams, B.P. et al. (1988) Immunogenetics. 27:322. This application was not tested by R&D Systems.

 
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Immunocytochemistry
8-25 µg/mL
Immersion fixed THP‑1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

TRA‑1‑85/CD147 in THP‑1 Human Cell Line. TRA‑1‑85/CD147 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human TRA‑1‑85/CD147 Biotinylated Monoclonal Antibody (Catalog # BAM3195) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-85/CD147

The TRA-1-85 clone recognizes the cell surface expressed Oka blood group antigen in almost all human cell types (1). This antibody is frequently used to identify human cells.

References
  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Entrez Gene IDs
682 (Human)
Alternate Names
OKa Blood Group Antigen; TRA185; TRA-1-85

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