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Human TRA-1-85/CD147 Fluorescein-conjugated Antibody

Catalog #: FAB3195F
2 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3195F
Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-85/CD147 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA‑1‑85/CD147 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3195F) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References
  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs
682 (Human)
Alternate Names
OKa Blood Group Antigen; TRA185; TRA-1-85

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Citations for Human TRA-1-85/CD147 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016-03-30;2016(0):7513252.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
    Authors: Chang C, Lai Y, Lamb L, Townes T
    PLoS ONE, 2014-05-14;9(5):e97335.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human TRA-1-85/CD147 Fluorescein-conjugated Antibody
By Anonymous on 06/10/2019
Application: Flow Sample Tested: Brain tumor Species: Human