Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

Catalog #: FAB6264P Datasheet / COA / SDS

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FAB6264P

Detection of TRANCE/TNFSF11/RANK L in Human PBMCs by Flow Cytometry.

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Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRANCE/TNFSF11/RANK L in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 685857

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Gly64-Asp245
Accession # AAC51762

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TRANCE/TNFSF11/RANK L in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human IL-2 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1020A) and either (A) Mouse Anti-Human TRANCE/TNFSF11/RANK L PE-conjugated Monoclonal Antibody (Catalog # FAB6264P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of TRANCE/TNFSF11/RANK L in HEK293 human cell line transfected with human TRANCE by Flow Cytometry. Human HEK293 cell line transfected with human TRANCE was stained with Mouse Anti-Human TRANCE/TNFSF11/RANK L PE-conjugated Monoclonal Antibody (Catalog # FAB6264P, filled histogram) or Mouse IgG2B PE Isotype Control(Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANK L, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2, aa corresponding to 1-73 in isoform 1 are missing. In isoform 3, aa 1-47 are missing. Human TRANCE ECD is 83% identical to mouse TRANCE ECD. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Long Name

TNF-related Activation-induced Cytokine

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Alternate Names

CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11