Human Transglutaminase 3/TGM3 Antibody

Catalog #: AF4604 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4604
AF4604-SP

Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Transglutaminase 3/TGM3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3
Ala2-Glu693
Accession # NP_003236

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot. Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Transglutaminase 3/TGM3 (Catalog # 4604-TG) (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Transglutaminase 3/TGM3 in Human Esophagus. Transglutaminase 3/TGM3 was detected in immersion fixed paraffin-embedded sections of human esophagus using Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the papillae layer in mucosa. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 3/TGM3

Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).

References

Kim, I.G. et al. (1993) J. Biol. Chem. 268:12682.
Griffin, M. et al. (2002) Biochem. J. 368:377.
Lorand, L. and R.M. Graham (2003) Nat. Rev. Mol. Cell Biol. 4:140.
Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
Sardy, M. et al. (2002) J. Exp. Med. 195:747.

Entrez Gene IDs

7053 (Human); 21818 (Mouse)

Alternate Names

EC 2.3.2.13; MGC126250; protein-glutamine gamma-glutamyltransferase E; TG(E); TG3; TGase E; TGase-3; TgaseE; TGEMGC126249; TGM3; transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 3; Transglutaminase E; transglutaminase-3