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Human TROP-2 Antibody

Catalog #: AF650
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF650
AF650-SP
Detection of Human TROP‑2 by Western Blot.
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Product Details
Citations (20)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TROP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2
Thr88-Thr274
Accession # P09758
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TROP‑2 antibody by Western Blot. View Larger

Detection of Human TROP‑2 by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP-2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of TROP-2 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry TROP‑2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

TROP‑2 in Human Brain. TROP-2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References
  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

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Citations for Human TROP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Rescue of secretion of a rare-disease associated mis-folded mutant glycoprotein in UGGT1 knock-out mammalian cells
    Authors: Tax, G;Guay, KP;Soldà, T;Hitchman, CJ;Hill, JC;Vasiljevi?, S;Lia, A;Modenutti, CP;Straatman, KR;Santino, A;Molinari, M;Zitzmann, N;Hebert, DN;Roversi, P;Trerotola, M;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TROP-2 and 5hmC expression in follicular-patterned thyroid neoplasm emphasizing tiny well-formed papillae
    Authors: JY Seok, X Fan
    Annals of diagnostic pathology, 2022-01-31;57(0):151903.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release
    Authors: PR Kamble, SR Patkar, AA Breed, BR Pathak
    Archives of biochemistry and biophysics, 2021-11-10;714(0):109084.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  4. miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development
    Authors: Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan
    J Cancer, 2020-03-05;11(11):3375-3386.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Cervical carcinomas that overexpress human trophoblast cell-surface marker (Trop-2) are highly sensitive to the antibody-drug conjugate sacituzumab govitecan
    Authors: B Zeybek, A Manzano, A Bianchi, E Bonazzoli, S Bellone, N Buza, P Hui, S Lopez, E Perrone, P Manara, L Zammataro, G Altwerger, C Han, J Tymon-Rosa, G Menderes, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, A Santin
    Sci Rep, 2020-01-22;10(1):973.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Trop2 Promotes Multidrug Resistance by Regulating Notch1 Signaling Pathway in Gastric Cancer Cells
    Authors: X Kuai, L Jia, T Yang, X Huang, W Zhao, M Zhang, Y Chen, J Zhu, Z Feng, Q Tang
    Med. Sci. Monit., 2020-01-22;26(0):e919566.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  7. In�vitro and in�vivo activity of sacituzumab govitecan, an antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (Trop-2) in uterine serous carcinoma
    Authors: C Han, E Perrone, B Zeybek, S Bellone, J Tymon-Rosa, G Altwerger, G Menderes, J Feinberg, K Haines, ME Muller Kar, A Bianchi, L Zammataro, A Manzano, E Bonazzoli, P Manara, N Buza, P Hui, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, S Lopez, AD Santin
    Gynecol. Oncol., 2019-12-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. TROP-2 exhibits tumor suppressive functions in cervical cancer by dual inhibition of IGF-1R and ALK signaling
    Authors: STK Sin, Y Li, M Liu, S Ma, XY Guan
    Gynecol. Oncol., 2018-11-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Potential Novel Therapy Targets in Neuroendocrine Carcinomas of the Breast
    Authors: S Vranic, J Palazzo, S Sanati, E Florento, E Contreras, J Xiu, J Swensen, Z Gatalica
    Clin. Breast Cancer, 2018-09-05;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. TROP2 methylation and expression in tamoxifen-resistant breast cancer
    Authors: SM Zimmers, EP Browne, KE Williams, RM Jawale, CN Otis, SS Schneider, KF Arcaro
    Cancer Cell Int., 2018-07-06;18(0):94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Anti-Trop2 blockade enhances the therapeutic efficacy of ErbB3 inhibition in head and neck squamous cell carcinoma
    Authors: N Redlich, AM Robinson, KP Nickel, AP Stein, DL Wheeler, DR Adkins, R Uppaluri, RJ Kimple, BA Van Tine, LS Michel
    Cell Death Dis, 2018-01-05;9(1):5.
    Species: Xenograft
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  12. Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways
    Authors: H Guan, Z Guo, W Liang, H Li, G Wei, L Xu, H Xiao, Y Li
    BMC Cancer, 2017-07-14;17(1):486.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors.
    Authors: Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D
    Clin Cancer Res, 2015-05-05;21(17):3870-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis.
    Authors: Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L
    Oncol Lett, 2014-07-31;8(4):1670-1674.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Trop-2 is a determinant of breast cancer survival.
    Authors: Ambrogi, Federico, Fornili, Marco, Boracchi, Patrizia, Trerotola, Marco, Relli, Valeria, Simeone, Pasquale, La Sorda, Rossana, Lattanzio, Rossano, Querzoli, Patrizia, Pedriali, Massimo, Piantelli, Mauro, Biganzoli, Elia, Alberti, Saverio
    PLoS ONE, 2014-05-13;9(5):e96993.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  16. Upregulation of Trop-2 quantitatively stimulates human cancer growth.
    Authors: Trerotola, M, Cantanelli, P, Guerra, E, Tripaldi, R, Aloisi, A L, Bonasera, V, Lattanzio, R, de Lange, R, Weidle, U H, Piantelli, M, Alberti, S
    Oncogene, 2012-02-20;32(2):222-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody.
    Authors: Raji R, Guzzo F, Carrara L
    J. Exp. Clin. Cancer Res., 2011-11-10;30(0):106.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
    Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
    J. Invest. Dermatol., 2011-06-16;131(10):2049-57.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC-Fr
  19. Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
    Authors: Nakatsukasa M, Kawasaki S, Yamasaki K
    Am. J. Pathol., 2010-07-22;177(3):1344-55.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Immunoprecipitation
  20. Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers.
    Authors: Wang J, Day R, Dong Y, Weintraub SJ, Michel L
    Mol. Cancer Ther., 2008-02-01;7(2):280-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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