Human TRPS1 Antibody Summary
Met1-Phe222
Accession # Q9UHF7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TRPS1 by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRPS1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human TRPS1 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TRPS1 at approximately 191 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRPS1
TRPS1 (also GC79) is a 160-175 kDa (predicted 140 kDa) member of the zinc finger family of transcription factors. It is ubiquitously expressed and serves to repress the expression of GATA-regulated genes. Human TRPS1 is 1281 amino acids (aa) in length. It contains nine divergent zinc-finger motifs (aa 222‑1267), the seventh of which is associated with a GATA-binding sequence (aa 896‑920) and is flanked by two NLSs (aa 886‑891 and 946‑952). Two SUMOylation sites exist (Lys1192 and Lys1201) and when utilized, they each add 40 kDa to the native MW. One alternative splice form shows premature truncation after Lys853. A second shows an alternate start site 13 aa upstream of the standard start site. Over aa 1‑222, human TRPS1 shares 95% and 89% aa identity with canine and mouse TRPS1, respectively.
Product Datasheets
Citations for Human TRPS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer
Authors: J Zhang, X Lv, B Wei, X Gong, L Chen
Cellular Signalling, 2022-09-06;100(0):110464.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Atypical GATA transcription factor TRPS1 represses gene expression by recruiting CHD4/NuRD(MTA2) and suppresses cell migration and invasion by repressing TP63 expression
Authors: Y Wang, X Lin, X Gong, L Wu, J Zhang, W Liu, J Li, L Chen
Oncogenesis, 2018-12-19;7(12):96.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation
Authors: X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen
J. Biol. Chem., 2018-10-12;0(0):.
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Tricho-rhino-phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin-specific protease 4-directed histone deacetylase 2 de-ubiquitination and tumor growth
Authors: Y Wang, J Zhang, L Wu, W Liu, G Wei, X Gong, Y Liu, Z Ma, F Ma, JP Thiery, L Chen
Breast Cancer Res., 2018-08-02;20(1):83.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TRPS1 regulates oestrogen receptor binding and histone acetylation at enhancers
Authors: AA Serandour, H Mohammed, A Miremadi, KW Mulder, JS Carroll
Oncogene, 2018-06-12;0(0):.
Species: Human
Sample Types: Cell Lysates, Complex Sample Type
Applications: ChIP, Western Blot
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https://doi.org/10.1038/s41388-018-0312-2
