Human VE-Cadherin Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 13.4 | 34.6 | 65.4 | 12.8 | 31.8 | 64.5 |
| Standard Deviation | 0.68 | 1.63 | 2.05 | 0.92 | 1.64 | 2.86 |
| CV% | 5.1 | 4.7 | 3.1 | 7.2 | 5.1 | 4.4 |
Recovery
The recovery of VE-Cadherin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-108 |
Linearity
Scientific Data
Preparation and Storage
Background: VE-Cadherin
The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human VE-Cadherin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin
Authors: PN Kouam, GA Rezniczek, IA Adamietz, H Bühler
BMC Cancer, 2019-10-16;19(1):958.
Species: Human
Sample Types: Cell Culture Supernates
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Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system
Authors: NJ Aachmann-A, SJ Christense, K Lisbjerg, P Oturai, PI Johansson, NH Holstein-R, NV Olsen
Physiol Rep, 2018-03-01;6(5):.
Species: Human
Sample Types: Serum
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How biomarkers reflect the prognosis and treatment of necrotising soft tissue infections and the effects of hyperbaric oxygen therapy: the protocol of the prospective cohort PROTREAT study conducted at a tertiary hospital in Copenhagen, Denmark
Authors: P Polzik, PI Johansson, O Hyldegaard
BMJ Open, 2017-10-05;7(10):e017805.
Species: Human
Sample Types: Plasma
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Association of Endothelial Glycocalyx and Tight and Adherens Junctions With Severity of Plasma Leakage in Dengue Infection
Authors: S Suwarto, RT Sasmono, R Sinto, E Ibrahim, M Suryamin
J. Infect. Dis., 2017-03-15;215(6):992-999.
Species: Human
Sample Types: Serum
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