Human VEGF-D Biotinylated Antibody

Catalog #: BAF286 Datasheet / COA / SDS

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BAF286

All VEGF-D Antibodies

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Human VEGF-D Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF-D in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant mouse VEGF-D (under non‑reducing conditions) is observed and less than 1% cross-reactivity with recombinant human (rh) VEGF and rhVEGF-B is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VEGF-D
Phe93-Ser201
Accession # O43915

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human VEGF-D (Catalog # 622-VD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-D

Vascular endothelial growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cystine knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C‑terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non‑covalently linked 42 kDa homodimers of the 117 aa VHD (4‑6). Mature human VEGF-D shares 94%, 95%, 99%, 97%, and 93% aa identity with mouse, rat, equine, canine and bovine VEGF-D, respectively (4, 5). It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF Receptor 3 (VEGF R3; also called Flt-4) that are active across species and show enhanced affinity when processed (7). Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR (7). VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (8‑10). Both promote tumor lymphangiogenesis (11). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (12, 13).

References

Roy, H. et al. (2006) FEBS Lett. 580:2879.
Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258.
Yamada, Y. et al. (1997) Genomics 42:483.
Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127.
McColl, B.K. et al. (2003) J. Exp. Med. 198:863.
McColl, B.K. et al. (2007) FASEB J. 21:1088.
Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166.
Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441.
Karpanen, T. et al. (2006) Am. J. Pathol. 169:708.
Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961.
Stacker, S.A. et al. (2001) Nature Med. 7:186.
Karpanen, T. et al. (2006) FASEB J. 20:1462.
Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.

Long Name

Vascular Endothelial Growth Factor D/cFos-induced Growth Factor

Entrez Gene IDs

2277 (Human); 14205 (Mouse)

Alternate Names

c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor