Human VEGF PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of VEGF in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line either treated with 1x Cell Activation Cocktail overnight (filled histogram; 5476) or untreated (open histogram) was stained with Mouse Anti-Human VEGF PE-conjugated Monoclonal Antibody (Catalog # IC2931P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF
Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.
Product Datasheets
Citations for Human VEGF PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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In vitro angiogenesis inhibition with selective compounds targeting the key glycolytic enzyme PFKFB3
Authors: Anahita Abdali, Denisa Baci, Isabella Damiani, Federica Belloni, Carlo De Dominicis, Maria Luisa Gelmi et al.
Pharmacological Research
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Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition
Authors: Oladapo O. Yeku, Thapi Dharma Rao, Ian Laster, Artem Kononenko, Terence J. Purdon, Pei Wang et al.
Frontiers in Immunology
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Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer
Authors: E Leveque, A Rouch, C Syrykh, J Mazières, L Brouchet, S Valitutti, E Espinosa, F Lafouresse
Cancers, 2022-03-09;14(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance
Authors: S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood
Cell Reports, 2021-08-17;36(7):109549.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intratumor Regulatory Noncytotoxic NK Cells in Patients with Hepatocellular Carcinoma
Authors: A Zecca, V Barili, D Rizzo, A Olivani, E Biasini, D Laccabue, R Dalla Vall, C Ferrari, E Cariani, G Missale
Cells, 2021-03-10;10(3):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater
Authors: D Baci, M Gallazzi, C Cascini, M Tramacere, D De Stefano, A Bruno, DM Noonan, A Albini
Int J Mol Sci, 2019-01-14;20(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
Authors: Lee HS, Myers A, Kim J
Am. J. Respir. Crit. Care Med., 2009-09-17;180(11):1056-67.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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