Human VEGF/PlGF Heterodimer Antibody

Catalog #: MAB297 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB297
MAB297-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGF/PlGF Heterodimer Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF/PlGF heterodimer in direct ELISAs and Western blots. This antibody does not detect either subunit alone. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF₁₂₁, rhVEGF₁₆₅, rhVEGF₂₀₆, rhCTGF, rhLDGF, rhPlGF, rhVEGF‑B₁₈₆, rhVEGF‑C, rhVEGF‑D, rhPDGF, rmVEGF₁₁₅, rmVEGF₁₂₀, rmVEGF₁₆₄, rmPlGF‑2, rrVEGF₁₆₄, or rzfVEGF.

Source

Monoclonal Mouse IgG_2A Clone # 146213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VEGF/PlGF heterodimer
Ala27-Arg191 (VEGF) & Ala21-Arg149 (PIGF)
Accession # AAM03108 (VEGF) & AAD30179 (PIGF)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human VEGF/PlGF Heterodimer (Catalog # 297-VP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF/PlGF Heterodimer

The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.

Long Name

Vascular Endothelial Growth Factor/Placenta Growth Factor Heterodimer

Alternate Names

VEGF/PlGF Heterodimer