Human VEGFR1/Flt-1 APC-conjugated Antibody

Catalog #: FAB321A
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB321A

Detection of VEGF R1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.

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All VEGFR1/Flt-1 Antibodies

Product Details

Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR1/Flt-1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.

Source

Monoclonal Mouse IgG₁ Clone # 49560

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
Ser27-His687
Accession # NP_001153392

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VEGF R1/Flt‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R1/Flt-1 APC-conjugated Monoclonal Antibody (Catalog # FAB321A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human VEGFR1/Flt-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Cytotoxic effects and tolerability of gemcitabine and axitinib in a xenograft model for c-myc amplified medulloblastoma
Authors: S Schwinn, Z Mokhtari, S Thusek, T Schneider, AL Sirén, N Tiemeyer, I Caruana, E Miele, PG Schlegel, A Beilhack, M Wölfl
Scientific Reports, 2021-07-07;11(1):14062.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
BMC Cancer, 2014-12-12;14(0):939.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Neutrophil expression of ICAM1, CXCR1, and VEGFR1 in patients with breast cancer before and after adjuvant chemotherapy.
Authors: Lohri C, Schaltegger C, van den Broek M, Wenger R, Ruegg C, Fink D, Fehr M, Knuth A, Zweifel M
Anticancer Res, 2014-09-01;34(9):4693-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
Authors: Tannetta D, Dragovic R, Gardiner C, Redman C, Sargent I
PLoS ONE, 2013-02-20;8(2):e56754.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept.
Authors: de Groot JF, Piao Y, Tran H, Gilbert M, Wu HK, Liu J, Bekele BN, Cloughesy T, Mehta M, Robins HI, Lassman A, Deangelis L, Camphausen K, Chen A, Yung W, Prados M, Wen PY, Heymach JV
Clin. Cancer Res., 2011-06-01;17(14):4872-4881.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
Authors: Xiong YQ, Sun HC, Zhang W
Clin. Cancer Res., 2009-07-28;15(15):4838-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Blood-based biomarkers of SU11248 activity and clinical outcome in patients with metastatic imatinib-resistant gastrointestinal stromal tumor.
Authors: Norden-Zfoni A, Desai J, Manola J, Beaudry P, Force J, Maki R, Folkman J, Bello C, Baum C, DePrimo SE, Shalinsky DR, Demetri GD, Heymach JV
Clin. Cancer Res., 2007-05-01;13(9):2643-50.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry