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Human Vitamin D BP ELISA Kit - Quantikine

Catalog #: DVDBP0B
35 Citations 1 Review Datasheet / COA / SDS
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QC185B: Quantikine Immunoassay Control Set 925 Human Vitamin D BP
Human Vitamin D BP ELISA Standard Curve
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Citations (35)
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Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Vitamin D BP Quantikine ELISA Kit Summary

Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
0.338 ng/mL
Assay Range
6.3 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human Vitamin D BP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Vitamin D BP immunoassay is a 3.5 hour sandwich-type solid phase ELISA designed to measure human Vitamin D BP in cell culture supernates, serum, plasma, and urine. It contains HEK293-expressed recombinant human Vitamin D BP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Vitamin D BP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Vitamin D BP.

Read more about the Vitamin D BP specificity in this White Paper.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 10.9 31.7 63.7 10.1 30.8 61.9
Standard Deviation 0.244 0.423 1.36 0.661 0.664 1.31
CV% 2.2 1.3 2.1 6.5 2.2 2.1

Recovery

The recovery of human Vitamin D BP spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 93-117
Urine (n=4) 89 78-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Vitamin D BP ELISA Linearity
Human Vitamin D BP ELISA Linearity

Scientific Data

N/A Human Vitamin D BP ELISA Standard Curve View Larger

Human Vitamin D BP ELISA Standard Curve

N/A Human Vitamin D BP Standard Curve View Larger

Human Vitamin D BP Standard Curve

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Vitamin D BP

Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.

Long Name:
Vitamin D Binding Protein
Entrez Gene IDs:
2638 (Human); 14473 (Mouse); 24384 (Rat)
Alternate Names:
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Vitamin D BP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Unravelling the Contribution of the rs7041 and rs4588 Polymorphisms of the GC Gene and Serum VDBP Levels for Developing Metabolic Syndrome in the Mexican Population
    Authors: A Hidalgo-Br, B Rivera-Par, G León-Reyes, N Patiño, M Castillejo, J Salmerón, R Velázquez-
    International Journal of Molecular Sciences, 2022-09-13;23(18):.
    Species: Human
    Sample Types: Serum
  2. Interplay between Maternal and Neonatal Vitamin D Deficiency and Vitamin-D-Related Gene Polymorphism with Neonatal Birth Anthropometry
    Authors: SS Lee, KH Ling, M Tusimin, R Subramania, KF Rahim, SP Loh
    Nutrients, 2022-01-27;14(3):.
    Species: Human
    Sample Types: Plasma
  3. Vitamin-D Binding Protein Gene Polymorphisms and Serum 25-Hydroxyvitamin-D in a Turkish Population
    Authors: L Karc?o?lu, A Özayd?n, ME Mavi?, GG Gürsu, L Harbige, N Hekim
    Metabolites, 2021-10-12;11(10):.
    Species: Human
    Sample Types: Serum
  4. Total, Bioavailable, and Free 25-Hydroxyvitamin D Equally Associate with Adiposity Markers and Metabolic Traits in Mexican Adults
    Authors: B Rivera-Par, A Hidalgo-Br, G León-Reyes, LS León-Maldo, A Aquino-Gál, M Castillejo, E Denova-Gut, YN Flores, J Salmerón, R Velázquez-
    Nutrients, 2021-09-23;13(10):.
    Species: Human
    Sample Types: Serum
  5. Correlational analysis of bone health status and vitamin D-related biomarkers in women working in agriculture
    Authors: MC Cho, KS Park, JK Shin, SA Lee, IA Cho, HC Jo, SC Kim, WJ Choi
    Medicine, 2021-08-27;100(34):e27071.
    Species: Human
    Sample Types: Serum
  6. Association of GC Variants with Bone Mineral Density and Serum VDBP Concentrations in Mexican Population
    Authors: B Rivera-Par, A Hidalgo-Br, G León-Reyes, B Antuna-Pue, YN Flores, J Salmerón, R Velázquez-
    Genes, 2021-07-29;12(8):.
    Species: Human
    Sample Types: Serum
  7. Longitudinal changes in vitamin D and its metabolites in pregnant South Africans
    Authors: JA George, SA Norris, T Snyman, L Khambule, M Toman, NJ Crowther
    The Journal of steroid biochemistry and molecular biology, 2021-07-07;212(0):105949.
    Species: Human
    Sample Types: Plasma
  8. Genome-wide association study of vitamin D concentrations and bone mineral density in the African American-Diabetes Heart Study
    Authors: ND Palmer, L Lu, TC Register, L Lenchik, JJ Carr, PJ Hicks, SC Smith, J Xu, L Dimitrov, J Keaton, M Guan, MCY Ng, YI Chen, AJ Hanley, CD Engelman, JM Norris, CD Langefeld, LE Wagenknech, DW Bowden, BI Freedman, J Divers
    PLoS ONE, 2021-05-20;16(5):e0251423.
    Species: Human
    Sample Types: Plasma
  9. Association of SNPs in GC and CYP2R1 with total and directly measured free 25-hydroxyvitamin D in multi-ethnic postmenopausal women in Saudi Arabia
    Authors: S Alharazy, MI Naseer, E Alissa, MD Robertson, S Lanham-New, MH Alqahtani, AG Chaudhary
    Saudi journal of biological sciences, 2021-05-04;28(8):4626-4632.
    Species: Human
    Sample Types: Serum
  10. Total vs. Bioavailable: Determining a Better 25(OH)D Index in Association with Bone Density and Muscle Mass in Postmenopausal Women
    Authors: NZ Abidin, SR Mitra
    Metabolites, 2020-12-31;11(1):.
    Species: Human
    Sample Types: Plasma
  11. Lack of association between Vitamin D status and free light chains profile with different chronic HCV-related liver and extrahepatic disorders
    Authors: U Basile, C Napodano, K Pocino, A Barini, M Marino, SA Santini, A Barini, A Stefanile, V Basile, CA Callà, P Cattani, A Gasbarrini, GL Rapaccini, F Gulli
    Eur Rev Med Pharmacol Sci, 2019-10-01;23(19):8506-8514.
    Species: Human
    Sample Types: Serum
  12. Metabolomics in plasma of Malawian children 7?years after surviving severe acute malnutrition: "ChroSAM" a cohort study
    Authors: C Bourdon, N Lelijveld, D Thompson, PS Dalvi, GB Gonzales, D Wang, M Alipour, E Wine, E Chimwezi, JC Wells, M Kerac, R Bandsma, MJ Nyirenda
    EBioMedicine, 2019-06-27;0(0):.
    Species: Human
    Sample Types: Plasma
  13. Free Vitamin D: Relationship to Insulin Sensitivity and Vascular Health in Youth
    Authors: F Bacha, SK Bartz, A Tomsa, S Sharma
    J. Pediatr., 2019-06-11;0(0):.
    Species: Human
    Sample Types: Plasma
  14. Differential proteome analysis in adolescent idiopathic scoliosis patients with thoracolumbar/lumbar curvatures
    Authors: H Makino, S Seki, I Kitajima, H Motomura, M Nogami, Y Yahara, N Ejiri, T Kimura
    BMC Musculoskelet Disord, 2019-05-24;20(1):247.
    Species: Human
    Sample Types: Plasma
  15. Cord Blood Vitamin D Levels and Early Childhood Blood Pressure: The Healthy Start Study
    Authors: KA Sauder, AV Stamatoiu, E Leshchinsk, BM Ringham, DH Glueck, D Dabelea
    J Am Heart Assoc, 2019-05-07;8(9):e011485.
    Species: Human
    Sample Types: Serum
  16. Prognostic significance of vitamin D receptor (VDR) gene polymorphisms in liver cirrhosis
    Authors: C Triantos, I Aggeletopo, M Kalafateli, PI Spantidea, G Vourli, G Diamantopo, D Tapratzi, M Michalaki, S Manolakopo, C Gogos, V Kyriazopou, A Mouzaki, K Thomopoulo
    Sci Rep, 2018-09-14;8(1):14065.
    Species: Human
    Sample Types: Serum
  17. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein
    Authors: B Petrov, A Aldoori, C James, K Yang, GP Algorta, A Lee, L Zhang, T Lin, RA Awadhi, JR Parquette, A Samogyi, LE Arnold, MA Fristad, B Gracious, O Ziouzenkov
    Transl Psychiatry, 2018-03-13;8(1):61.
    Species: Human
    Sample Types: Serum
  18. Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status
    Authors: Yeo Min Yun
    Ann Lab Med, 2017-01-01;37(1):34-38.
    Species: Human
    Sample Types: Serum
  19. Bioavailable 25(OH)D but Not Total 25(OH)D Is an Independent Determinant for Bone Mineral Density in Chinese Postmenopausal Women
    Authors: C Li, P Chen, X Duan, J Wang, B Shu, X Li, Q Ba, J Li, Y Wang, H Wang
    EBioMedicine, 2016-11-28;15(0):184-192.
    Species: Human
    Sample Types: Plasma
  20. Genetically Low Vitamin D Levels, Bone Mineral Density, and Bone Metabolism Markers: a Mendelian Randomisation Study
    Sci Rep, 2016-09-14;6(0):33202.
    Species: Human
    Sample Types: Serum
  21. Pharmacokinetics and effects of demographic factors on blood 25(OH)D3 levels after a single orally administered high dose of vitamin D3
    Acta Pharmacol Sin, 2016-08-29;0(0):.
    Species: Human
    Sample Types: Plasma
  22. Urinary Vitamin D Binding Protein and KIM-1 Are Potent New Biomarkers of Major Adverse Renal Events in Patients Undergoing Coronary Angiography.
    Authors: Chaykovska L, Heunisch F, von Einem G, Alter M, Hocher C, Tsuprykov O, Dschietzig T, Kretschmer A, Hocher B
    PLoS ONE, 2016-01-11;11(1):e0145723.
    Species: Human
    Sample Types: Plasma
  23. Serum vitamin D levels are positively associated with varicella zoster immunity in chronic dialysis patients.
    Authors: Chao C, Lee S, Yang W, Yen C, Chiang C, Huang J, Hung K
    Sci Rep, 2014-12-09;4(0):7371.
    Species: Human
    Sample Types: Serum
  24. Associations between vascular health indices and serum total, free and bioavailable 25-hydroxyvitamin D in adolescents.
    Authors: Ashraf A, Alvarez J, Dudenbostel T, Calhoun D, Griffin R, Wang X, Hanks L, Gower B
    PLoS ONE, 2014-12-05;9(12):e114689.
    Species: Human
    Sample Types: Serum
  25. Serum vitamin D, vitamin D binding protein, and risk of colorectal cancer.
    Authors: Anic G, Weinstein S, Mondul A, Mannisto S, Albanes D
    PLoS ONE, 2014-07-18;9(7):e102966.
    Species: Human
    Sample Types: Serum
  26. Vitamin D-binding protein, circulating vitamin D and risk of renal cell carcinoma.
    Authors: Mondul A, Weinstein S, Moy K, Mannisto S, Albanes D
    Int J Cancer, 2014-01-30;134(11):2699-706.
    Species: Human
    Sample Types: Serum
  27. A bone substitute with high affinity for vitamin D-binding protein--relationship with niche of osteoclasts.
    Authors: Ikeda T, Kasai M, Tatsukawa E, Kamitakahara M, Shibata Y, Yokoi T, Nemoto T, Ioku K
    J Cell Mol Med, 2013-11-28;18(1):170-80.
    Species: Human
    Sample Types: Serum
  28. Circulating 25-hydroxyvitamin D, vitamin D-binding protein and risk of prostate cancer.
    Authors: Weinstein S, Mondul A, Kopp W, Rager H, Virtamo J, Albanes D
    Int J Cancer, 2012-12-27;132(12):2940-7.
    Species: Human
    Sample Types: Serum
  29. Vitamin D in a northern Canadian first nation population: dietary intake, serum concentrations and functional gene polymorphisms.
    Authors: Larcombe L, Mookherjee N, Slater J, Slivinski C, Singer M, Whaley C, Denechezhe L, Matyas S, Turner-Brannen E, Nickerson P, Orr P
    PLoS ONE, 2012-11-21;7(11):e49872.
    Species: Human
    Sample Types: Serum
  30. Influence of vitamin D binding protein on the association between circulating vitamin D and risk of bladder cancer.
    Br. J. Cancer, 2012-09-18;107(9):1589-94.
    Species: Human
    Sample Types: Serum
  31. Bioavailable vitamin D is more tightly linked to mineral metabolism than total vitamin D in incident hemodialysis patients.
    Authors: Bhan I, Powe CE, Berg AH
    Kidney Int., 2012-03-07;82(1):84-9.
    Species: Human
    Sample Types: Serum
  32. Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration.
    Authors: Heijboer AC, Blankenstein MA
    Clin. Chem., 2012-01-12;58(3):543-8.
    Species: Human
    Sample Types: Plasma
  33. Vitamin D supplementation during pregnancy: double-blind, randomized clinical trial of safety and effectiveness.
    Authors: Hollis BW, Johnson D, Hulsey TC, Ebeling M, Wagner CL
    J. Bone Miner. Res., 2011-10-01;26(10):2341-57.
    Species: Human
    Sample Types: Serum
  34. Oral cholecalciferol decreases albuminuria and urinary TGF-beta1 in patients with type 2 diabetic nephropathy on established renin-angiotensin-aldosterone system inhibition.
    Authors: Kim MJ, Frankel AH, Donaldson M, Darch SJ, Pusey CD, Hill PD, Mayr M, Tam FW
    Kidney Int., 2011-08-10;80(8):851-60.
    Species: Human
    Sample Types: Urine
  35. Differences in bone metabolism between singleton pregnancy and twin pregnancy.
    Authors: Nakayama S, Yasui T, Suto M, Sato M, Kaji T, Uemura H, Maeda K, Irahara M
    Bone, 2011-05-27;49(3):513-9.
    Species: Human
    Sample Types: Serum

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Human Vitamin D BP Quantikine ELISA Kit
By Anonymous on 02/19/2022
Sample Tested: A549 human lung carcinoma cell line
Human Vitamin D BP Quantikine ELISA Kit DVDBP0B