Human VSIG1 Antibody Summary
Val22-Gly234
Accession # Q86XK7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human VSIG1 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VSIG1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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VSIG1 in Human Testis. VSIG1 was detected in immersion fixed frozen sections of human testis using 10 µg/mL Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Detection of Human VSIG1 by Simple WesternTM. Simple Western lane view shows lysates of human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for VSIG1 at approximately 115 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG1
VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.
Product Datasheets
Citation for Human VSIG1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers.
Authors: Delker D, McGettigan B, Kanth P, Pop S, Neklason D, Bronner M, Burt R, Hagedorn C
PLoS ONE, 2014-02-12;9(2):e88367.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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