Human VSIG3 Antibody

Catalog # Availability Size / Price Qty
MAB92293-100
MAB92293-SP
Detection of VSIG3 in HEK293 Human Cell Line transfected with human VSIG3 and eGFP by Flow Cytometry.
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Human VSIG3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VSIG3 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 973422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line transfected with human VSIG3 and eGFP
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VSIG3 antibody in HEK293 Human Cell Line transfected with human VSIG3 and eGFP antibody by Flow Cytometry. View Larger

Detection of VSIG3 in HEK293 Human Cell Line transfected with human VSIG3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human VSIG3 monoclonal antibody (Catalog # MAB92293) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Blockade of Receptor-ligand Interaction View Larger

VSIG3 Binding to VISTA/B7-H5/PD-1H by Human VISG3 Antibody. In a functional ELISA, 0.2-2 µg/mL of Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92293) will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG3

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Long Name
V-Set and Immunoglobulin Domain Containing 3
Entrez Gene IDs
152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)
Alternate Names
Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein

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