Human VSIG3 Antibody Summary
Met1-Gly245
Accession # Q5DX21
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of VSIG3 in HEK293 Human Cell Line transfected with human VSIG3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human VSIG3 monoclonal antibody (Catalog # MAB92293) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.
VSIG3 Binding to VISTA/B7-H5/PD-1H by Human VISG3 Antibody. In a functional ELISA, 0.2-2 µg/mL of Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92293) will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG3
VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Product Datasheets
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