Human VSIG4 Antibody

Catalog #: MAB4646 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4646-100
MAB4646-SP

Detection of Human VSIG4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VSIG4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 528903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human VSIG4 protein
Arg20-Pro283
Accession # Q9Y279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HEK293T human embryonic kidney cell line transfected with human VSIG4

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC-derived macrophages

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human VSIG4 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human VSIG4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB4646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of VSIG4 in Human Macrophages by Flow Cytometry Human CD14+ monocytes were positively selected from PBMC (MAGH105) and cultured for 7 days with 10% Human AB serum, 50 ng/ml M-CSF (216-MC), and 10μM hydrocortisone (4093). Cells were harvested and stained with either (A) Mouse anti-Human VSIG4 (Catalog # MAB4646) or (B) Isotype control antibody (MAB003) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB16991G). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG4

VSIG4 (Vset and immunoglobulin domain containing 4), also known as complement receptor immunoglobulin (CRIg) and Z39IG, is a 45 kDa, type I transmembrane protein of the B7 family within the Ig superfamily that is expressed only in tissue resident macrophages (1-4). The gene is located on the X chromosome (2). The human VSIG4 cDNA encodes 399 amino acids (aa) including a 19 aa signal sequence, a 264 aa extracellular domain (ECD) containing a V-type and a C2-type Ig domain, a 21 aa transmembrane domain and a 95 aa cytoplasmic domain (3). The human VSIG4 ECD shares 84% aa identity with canine VSIG4. Within the IgV domain, it shares 90%, 80% and 78% aa identity with bovine, mouse and rat VSIG4, respectively; these animals lack the C2-type domain. Splice isoforms of 321, 305, 272, 201 and 199 aa lack all or part of the cytoplasmic domain, the C2-type Ig domain and/or the transmembrane domain (5). VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-4, 6-9). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 6). It contributes significantly to innate immunity by binding and phagocytosis of complement opsonized invading pathogens (4, 8, 10). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (10, 11). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappa B and thus be proinflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 7, 10, 11). There is emerging evidence in human conditions that VSIG4 may be a valuable biomarker in infection and immunity, inflammatory conditions and cancer prognosis (12, 13, 14).

References

He, J.Q. et al. (2008) Mol. Immunol. 4041.
Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
Helmy, K. et al. (2006) Cell 124:915.
Entrez protein Accession # EAX05393, NP_001093901, CAI42052, CAI4205, EAX05394.
Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
Lee, M.Y. et al. (2006) J. Leukoc. Biol. 80:922.
Gorgani, N.N. et al. (2008) J. Immunol. 181:7902.
Walker, M.G. (2002) Biochim. Biophys. Acta 1574:387.
Wiesmann, C. et al. (2006) Nature 444:217.
Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.
Small, A.G. et al. (2016) Swiss Med Wkly. 5:146.
Roh J. et al. (2017) Oncotarget. 8:58122.
Kim K.H. et al. (2016) Autophagy. 12:1647.

Long Name

V-Set and Immunoglobulin Domain Containing 4

Entrez Gene IDs

11326 (Human); 278180 (Mouse); 312102 (Rat); 102144784 (Cynomolgus Monkey)

Alternate Names

CRIg; Ig superfamily protein; Protein Z39Ig; V-set and immunoglobulin domain containing 4; V-set and immunoglobulin domain-containing protein 4; VSIG4; Z39IG; Z39IGcomplement receptor of the immunoglobulin superfamily