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Human XBP1 (S Isoform) Antibody

Catalog #: MAB4257
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4257
MAB4257-SP
Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry.
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Citations (2)
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Human XBP1 (S Isoform) Antibody Summary

Species Reactivity
Human
Specificity
Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).
Source
Monoclonal Mouse IgG1 Clone # 525904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human XBP1S
Ser188-Leu287
Accession # NP_001073007
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of XBP1S antibody in RPMI 8226 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XBP1

X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.

Long Name
X-box Binding Protein 1
Entrez Gene IDs
7494 (Human); 22433 (Mouse); 289754 (Rat)
Alternate Names
HTF; TREB5; X-box binding protein 1; X-box-binding protein 1; XBP1; XBP-1; XBP2; XBP2Tax-responsive element-binding protein 5

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Citations for Human XBP1 (S Isoform) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Induction of autophagy-dependent apoptosis in cancer cells through activation of ER stress: an uncovered anti-cancer mechanism by anti-alcoholism drug disulfiram
    Authors: X Zhang, P Hu, SY Ding, T Sun, L Liu, S Han, AB DeLeo, A Sadagopan, W Guo, X Wang
    Am J Cancer Res, 2019-06-01;9(6):1266-1281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. In vivo crystallization of human IgG in the endoplasmic reticulum of engineered Chinese hamster ovary (CHO) cells.
    Authors: Hasegawa H, Wendling J, He F
    J. Biol. Chem., 2011-04-04;286(22):19917-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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