Human ZNF10 Antibody

Name: Human ZNF10 Antibody
Brand: R&D Systems
SKU: MAB96561
Rating: 5 (1 reviews)

Catalog #: MAB96561 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB96561-100
MAB96561-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZNF10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZNF10 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 980803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human ZNF10
Accession # P21506

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ZNF10 in Human Tonsil. ZNF10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human ZNF10 Monoclonal Antibody (Catalog # MAB96561) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF10

Human Zinc finger protein ZNF10 (or KOX1) is a 573 amino acids protein encoded by the ZNF10 gene. ZNF10 contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor via the Kruppel-associated box (KRAB) domain of the protein. ZNF10 has been shown to interact with the nuclear hub protein TRIM28 to mediate chromatin-dependent processes like transcriptional repression, imprinting or suppression of endogenous retroviruses, representing a potential intrinsic antiretroviral defense.

Long Name

Zinc Finger Protein 10

Entrez Gene IDs

7556 (Human)

Alternate Names

KOX1; KOX1zinc finger protein 10 (KOX 1); zinc finger protein 10; Zinc finger protein KOX1; ZNF10