Human ZNF10 Antibody

Catalog #: MAB9656 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9656-100
MAB9656-SP

Detection of Human ZNF10 by Western Blot.

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Human ZNF10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZNF10 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 980809

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human ZNF10
Accession # P21506

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ZNF10 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, HeLa human cervical epithelial carcinoma cell line, human kidney tissue, and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ZNF10 Monoclonal Antibody (Catalog # MAB9656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF10 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF10

Human Zinc finger protein ZNF10 (or KOX1) is a 573 amino acids protein encoded by the ZNF10 gene. ZNF10 contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor via the Kruppel-associated box (KRAB) domain of the protein. ZNF10 has been shown to interact with the nuclear hub protein TRIM28 to mediate chromatin-dependent processes like transcriptional repression, imprinting or suppression of endogenous retroviruses, representing a potential intrinsic antiretroviral defense.

Long Name

Zinc Finger Protein 10

Entrez Gene IDs

7556 (Human)

Alternate Names

KOX1; KOX1zinc finger protein 10 (KOX 1); zinc finger protein 10; Zinc finger protein KOX1; ZNF10