Influenza A Virus H1N1 Hemagglutinin Antibody

Catalog #: MAB11396 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11396-100
MAB11396-SP

Detection of Hemagglutinin in Transfected CHO cells (positive) and Wild type CHO cells (negative).

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Influenza A Virus H1N1 Hemagglutinin Antibody Summary

Species Reactivity

Influenza A Virus H1N1

Specificity

Detects Influenza A Virus H1N1 Hemagglutinin in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 2908B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived influenza a virus H1N1 Hemagglutinin with a C-terminal 6-His tag
Met1-Gln529
Accession # YP_009118626

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

rvInflluenza A H1N1 Hemagglutinin

Immunocytochemistry

3-15 µg/mL

Immersion fixed Transfected CHO Chinese hamster ovary cells (positive) and Wild type CHO Chinese hamster ovary cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Hemagglutinin in Transfected CHO cells (positive) and Wild type CHO cells (negative). Hemagglutinin was detected in immersion fixed Transfected CHO Chinese hamster ovary cells (positive) and absent in Wild type CHO Chinese hamster ovary cells (negative) using Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB11396) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Influenza A Virus H1N1 Hemagglutinin by Western Blot. Western blot shows lysates of rvInflluenza A H1N1 Hemagglutinin. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB11396) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Hemagglutinin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Recombinant Hemagglutinin by Simple Western^TM. Simple Western lane view shows lysates of recombinant protein, loaded at 0.2 mg/ml. A specific band was detected for Hemagglutinin at approximately 111 kDa (as indicated) using 25 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (MAB11396). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemagglutinin

Influenza hemagglutinin (HA) is a homotrimeric glycoprotein found on the surface of influenza viruses. HA is responsible for binding influenza virus to sialic acid on the surface of target cells. HA is also responsible for the fusion of the viral envelope with the late endosomal protein when it is exposed to low pH. H1N1 is a subtype of Hemagglutinin in influenza A which also includes Neurominidase protein on its surface.

References

Russell, R.J. Kerry, P.S. Stevens, D.J. Steinhauer, D.A. Martin, S.R. Gamblin, S.H. Skehel, J.J. (November, 2008). "Structure of Invluenza Hemagllutinin in Complex with an Inhibitor of Membrane Fusion". Proceedings of the National Academy of Sciences of the United States of America. 105(46):17736-41.
Edinger, T.O. Pohl, O. Sertz, S. (February, 2014). "Entry of Influenza A. Virus: Host Factors and Antiviral Targets" (PDF). The Journal of General Virology. 95(Pt 2):263-277.
Horvath, P. Helenius, A. Yamauchi, Y. Banerjee, I. 12, July 2013. "High-Content Analysis of Sequential Events during the Early Phase of Influenza A Virus Infection". PLOS ONE. 8(7):e68450.
"Influenza Type A Viruses". Avian Influenza (Flu). CDC. 19, April 2017. Retrieved 27, August, 2018.

Long Name

Hemagglutinin

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Alternate Names

HA; Hemagglutinin