Influenza A Virus H1N1 Hemagglutinin Antibody

Catalog #: MAB113961 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB113961-100
MAB113961-SP

Detection of Influenza A Virus H1N1 Hemagglutinin by Western Blot.

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Influenza A Virus H1N1 Hemagglutinin Antibody Summary

Species Reactivity

Influenza A Virus H1N1

Specificity

Detects Influenza A Virus H1N1 Hemagglutinin in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 2908F

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

rvInflluenza A H1N1 Hemagglutinin

Simple Western

25 µg/mL

rvInfluenza A H1N1 Hemagluttinin

Immunocytochemistry

3-15 µg/mL

Immersion fixed CHO transfected cells (positive) and absent in Wild type CHO (negative) cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Influenza A Virus H1N1 Hemagglutinin by Western Blot. Western blot shows lysates of rvInflluenza A H1N1 Hemagglutinin. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Hemagglutinin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1

Immunocytochemistry

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Detection of Hemagglutinin in CHO transfected cells (positive) and Wild type CHO (negative) cells. Hemagglutinin was detected in immersion fixed CHO Chinese hamster ovary transfected cells (positive) and absent in Wild type CHO Chinese hamster ovary cells (negative) using Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Influenza A Virus H1N1 Hemagglutinin by Simple Western^TM. Simple Western lane view shows lysates of rvInfluenza A H1N1 Hemagluttinin, loaded at 200 ng/ml. A specific band was detected for Hemagglutinin at approximately 111 kDa (as indicated) using 25 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB113961). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Hemagglutinin

Influenza hemagglutinin (HA) is a homotrimeric glycoprotein found on the surface of influenza viruses. HA is responsible for binding influenza virus to sialic acid on the surface of target cells. HA is also responsible for the fusion of the viral envelope with the late endosomal protein when it is exposed to low pH. H1N1 is a subtype of Hemagglutinin in influenza A which also includes Neurominidase protein on its surface.

References

Russell, R.J. Kerry, P.S. Stevens, D.J. Steinhauer, D.A. Martin, S.R. Gamblin, S.H. Skehel, J.J. (November, 2008). "Structure of Invluenza Hemagllutinin in Complex with an Inhibitor of Membrane Fusion". Proceedings of the National Academy of Sciences of the United States of America. 105(46):17736-41.
Edinger, T.O. Pohl, O. Sertz, S. (February, 2014). "Entry of Influenza A. Virus: Host Factors and Antiviral Targets" (PDF). The Journal of General Virology. 95(Pt 2):263-277.
Horvath, P. Helenius, A. Yamauchi, Y. Banerjee, I. 12, July 2013. "High-Content Analysis of Sequential Events during the Early Phase of Influenza A Virus Infection". PLOS ONE. 8(7):e68450.
"Influenza Type A Viruses". Avian Influenza (Flu). CDC. 19, April 2017. Retrieved 27, August, 2018.

Long Name

Hemagglutinin

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Alternate Names

HA; Hemagglutinin