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Janelia Fluor® 549, free acid

Catalog #: 6503 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6503/5
Janelia Fluor® 549, free acid | CAS No. 2245946-45-4 | Free Acid Janelia Fluor Dyes
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Description: Yellow dye (Reactive group: free acid). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

Chemical Name: 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate

Purity: ≥90%

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Biological Activity

Janelia Fluor® 549, free acid is a yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Optical Data for Janelia Fluor® 549, free acid

Janelia Fluor<sup>®</sup> 549, free acid Dye Spectra
Emission Color Yellow
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Free acid
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

M.Wt:
568.5
Formula:
C27H22N2O5.C2HF3O2
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
2245946-45-4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

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