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Janelia Fluor® 630b, Maleimide

Catalog #: 8154 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8154/200U
Janelia Fluor® 630b, Maleimide | Sulfhydryl-Reactive Janelia Fluor Dyes
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Description: Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM

Chemical Name: N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3,7-bis(3-(methylsulfonyl)azetidin-1-yl)-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide

Purity: ≥90%

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Biological Activity

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 630b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: Excitation and emission maxima (λ) are 651 nM and 666 nM, respectively.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 630b, Maleimide

λabs 651 nm
λem 666 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
760.95
Formula:
C37H40N4O8S2Si
Purity:
≥90%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Reconstitution Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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