Methotrexate

Biological Activity

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs.
   Robinson T, Liu J, Vizeacoumar F, Sun T, Maclean N, Egan S, Schimmer A, Datti A, Zacksenhaus E
   PLoS ONE, 2013;8(11):e78641.
2. High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
   Fox JT, Sakamuru S, Huang R
   Proc. Natl. Acad. Sci. U.S.A., 2012;109(14):5423-8.
3. Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotr.
   Allegra et al.
   J.Biol.Chem., 1987;262:13520
4. Mechanism of thymidylate synthase inhibition by methotr. in human neoplastic cell lines and normal human myeloid progenitor cells.
   Chu et al.
   J.Biol.Chem., 1990;265:8470
5. The pharmacology and clinical use of methotr.
   Jolivet et al.
   N.Engl.J.Med., 1983;309:1094
6. Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotr.
   Winter-Vann et al.
   Proc.Natl.Acad.Sci.U.S.A., 2003;100:6529

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