Mouse ALCAM/CD166 Fluorescein-conjugated Antibody Summary
Trp28-Lys527
Accession # AAC06342
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172F) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108F). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: ALCAM/CD166
ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4). The ECD of mouse ALCAM shares 97.5% aa sequence identity with rat ALCAM ECD.
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Aruffo, A. et al. (1997) Immunol. Today 18:498.
- Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
- Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.
Product Datasheets
Citation for Mouse ALCAM/CD166 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Heterogeneity and dynamics of active Kras-induced dysplastic lineages from mouse corpus stomach
Authors: J Min, PN Vega, AC Engevik, JA Williams, Q Yang, LM Patterson, AJ Simmons, RJ Bliton, JW Betts, KS Lau, ST Magness, JR Goldenring, E Choi
Nat Commun, 2019-12-05;10(1):5549.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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