Mouse ALCAM/CD166 PE-conjugated Antibody

Catalog #: FAB1172P
3 Citations Datasheet / COA / SDS

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FAB1172P

Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry.

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All ALCAM/CD166 Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ALCAM/CD166 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172P) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4). The ECD of mouse ALCAM shares 97.5% aa sequence identity with rat ALCAM ECD.

References

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
Aruffo, A. et al. (1997) Immunol. Today 18:498.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Alternate Names

activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

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Related Research Areas

Citations for Mouse ALCAM/CD166 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Heterogeneity and dynamics of active Kras-induced dysplastic lineages from mouse corpus stomach
Authors: J Min, PN Vega, AC Engevik, JA Williams, Q Yang, LM Patterson, AJ Simmons, RJ Bliton, JW Betts, KS Lau, ST Magness, JR Goldenring, E Choi
Nat Commun, 2019-12-05;10(1):5549.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
Authors: KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo
Cell Stem Cell, 2017-07-06;21(1):78-90.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity.
Authors: Simonetti, Giorgia, Carette, Amanda, Silva, Kathryn, Wang, Haowei, De Silva, Nilushi, Heise, Nicole, Siebel, Christia, Shlomchik, Mark J, Klein, Ulf
J Exp Med, 2013-12-09;210(13):2887-902.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry