Mouse Alkaline Phosphatase/ALPL Antibody Summary
Phe18-Gly503
Accession # P09242
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of ALPL in Rat Stem Cells by Flow Cytometry. Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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Alkaline Phosphatase/ALPL in Rat Mesenchymal Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Alkaline Phosphatase/ALPL in Mouse Embryo. Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.
- Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
- Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Product Datasheets
Citations for Mouse Alkaline Phosphatase/ALPL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase
Authors: Yu, Y;Rong, K;Yao, D;Zhang, Q;Cao, X;Rao, B;Xia, Y;Lu, Y;Shen, Y;Yao, Y;Xu, H;Ma, P;Cao, Y;Qin, A;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Erythropoietin Receptor (EPOR) Signaling in the Osteoclast Lineage Contributes to EPO-Induced Bone Loss in Mice
Authors: Z Awida, S Hiram-Bab, A Bachar, H Saed, D Zyc, A Gorodov, N Ben-Califa, S Omari, J Omar, L Younis, JA Iden, L Graniewitz, I Gluzman, T Liron, B Raphael-Mi, A Kolomansky, M Rauner, B Wielockx, Y Gabet, D Neumann
International Journal of Molecular Sciences, 2022-10-10;23(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hyaluronidase 1 deficiency decreases bone mineral density in mice
Authors: E Puissant, F Gilis, V Tevel, JM Vandeweerd, B Flamion, M Jadot, M Boonen
Oncogene, 2022-06-16;12(1):10142.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration
Authors: S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang
Bioactive materials, 2022-03-24;18(0):116-127.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis
Authors: Y Chen, H Wang, Q Yang, W Zhao, Y Chen, Q Ni, W Li, J Shi, W Zhang, L Li, Y Xu, H Zhang, D Miao, L Xing, W Sun
Theranostics, 2022-01-01;12(3):1074-1096.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice
Authors: Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann
International Journal of Molecular Sciences, 2021-12-21;23(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG
Authors: LS Chen, M Zhang, P Chen, XF Xiong, PQ Liu, HB Wang, JJ Wang, J Shen
Acta pharmacologica Sinica, 2021-08-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine
Authors: Y Sun, JF Rahbani, MP Jedrychows, CL Riley, S Vidoni, D Bogoslavsk, B Hu, PA Dumesic, X Zeng, AB Wang, NH Knudsen, CR Kim, A Marasciull, JL Millán, ET Chouchani, L Kazak, BM Spiegelman
Nature, 2021-05-12;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance
Authors: X Yang, F Zhou, P Yuan, G Dou, X Liu, S Liu, X Wang, R Jin, Y Dong, J Zhou, Y Lv, Z Deng, S Liu, X Chen, Y Han, Y Jin
Bioactive materials, 2021-03-10;6(10):3150-3163.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation
Authors: S Yu, J Guo, Z Sun, C Lin, H Tao, Q Zhang, Y Cui, H Zuo, Y Lin, S Chen, H Liu, Z Chen
Cell Death & Disease, 2021-02-19;12(2):197.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
4-phenylbutyric acid enhances the mineralization of osteogenesis imperfecta iPSC-derived osteoblasts
Authors: S Takeyari, T Kubota, Y Ohata, M Fujiwara, T Kitaoka, Y Taga, K Mizuno, K Ozono
J Biol Chem, 2020-11-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
Authors: L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu
Stem Cell Res Ther, 2020-07-03;11(1):269.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells
Authors: G Voicu, D Rebleanu, CA Constantin, EV Fuior, L Ciortan, I Droc, CM Uritu, M Pinteala, I Manduteanu, M Simionescu, M Calin
Pharmaceutics, 2020-06-02;12(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A polysaccharide from the dried rhizome of Drynaria fortunei (Kunze) J. Sm. prevents ovariectomized (OVX)-induced osteoporosis in rats
Authors: X Sun, B Wei, Z Peng, X Chen, Q Fu, C Wang, J Zhen, J Sun
J. Cell. Mol. Med., 2020-02-17;0(0):.
Species: Rat
Sample Types: Serum
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Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
FEBS Open Bio, 2019-12-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits
Authors: L Yin, ZX Zhou, M Shen, N Chen, F Jiang, SL Wang
Stem Cells Int, 2019-12-11;2019(0):9845497.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Responses of primary osteoblasts and osteoclasts from hemizygous beta-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D3
Authors: N Charoenpha, R Aeimlapa, S Sooksawanw, J Thongbunch, J Teerapornp, S Svasti, K Wongdee
Sci Rep, 2019-09-27;9(1):13963.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
Cell Stem Cell, 2018-11-29;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia
Authors: W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin
Theranostics, 2018-11-09;8(20):5575-5592.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis
Authors: RM Sterner, KN Kremer, A Dudakovic, JJ Westendorf, AJ van Wijnen, KE Hedin
J. Immunol., 2018-06-18;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb girdle muscular dystrophy type 2E
Authors: G Giovannell, G Giacomazzi, H Grosemans, M Sampaolesi
Muscle Nerve, 2018-02-24;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification
Authors: Arjun Deb
Cell Stem Cell, 2016-11-17;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.
Authors: Yoshizaki, Keigo, Hu, Lizhi, Nguyen, Thai, Sakai, Kiyoshi, He, Bing, Fong, Chak, Yamada, Yoshihik, Bikle, Daniel D, Oda, Yuko
PLoS ONE, 2014-06-20;9(6):e99991.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
Authors: Isaac, J, Erthal, J, Gordon, J, Duverger, O, Sun, H-W, Lichtler, A C, Stein, G S, Lian, J B, Morasso, M I
Cell Death Differ, 2014-06-20;21(9):1365-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading.
Authors: Liedert A, Mattausch L, Rontgen V, Blakytny R, Vogele D, Pahl M, Bindl R, Neunaber C, Schinke T, Harroch S, Amling M, Ignatius A
Bone, 2010-12-23;48(4):945-51.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Mouse bone marrow cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody or followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
