Mouse Amphiregulin Biotinylated Antibody Summary
Applications
Mouse Amphiregulin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Amphiregulin
Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1-B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.
- Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
- Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
- Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.
Product Datasheets
Citations for Mouse Amphiregulin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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m6A RNA modification regulates innate lymphoid cell responses in a lineage-specific manner
Authors: Zhang, Y;Zhang, W;Zhao, J;Ito, T;Jin, J;Aparicio, AO;Zhou, J;Guichard, V;Fang, Y;Que, J;Urban, JF;Hanna, JH;Ghosh, S;Wu, X;Ding, L;Basu, U;Huang, Y;
Nature immunology
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
Authors: AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis
Nature, 2022-11-02;0(0):.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
gammadelta T cells and adipocyte IL-17RC control fat innervation and thermogenesis
Authors: B Hu, C Jin, X Zeng, JM Resch, MP Jedrychows, Z Yang, BN Desai, AS Banks, BB Lowell, D Mathis, BM Spiegelman
Nature, 2020-02-19;578(7796):610-614.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulatory T Cells Promote Myositis and Muscle Damage in Toxoplasma gondii Infection
Authors: Richard M Jin
J. Immunol, 2016-11-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Amphiregulin: an early trigger of liver regeneration in mice.
Authors: Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Lee DC, Prieto J, Avila MA
Gastroenterology, 2005-02-01;128(2):424-32.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
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Splenocytes (2x10^6) from a C57BL/6 mouse were stimulated for 4 hours with phorbol 12,13-dibutyrate (500ng/ml), ionomycin (750ng/ml) and brefeldin A (2ug/ml). Cells were stained with fixable viability dye, then fixed with 2% formalin, permeabilized, and stained overnight at 4C for cytokines and gating markers (CD3, CD4, Foxp3).
The biotinylated amphiregulin antibody was used at a 1:500 dilution overnight in permeabilization buffer. The cells were washed twice, then incubated with Brilliant Violet 605 streptavidin at a 1:1000 dilution for 20min.
Foxp3+ Tregs were chosen as a positive control. Other appropriate positive controls would be Th2 cells or ILC2 (e.g., gut lamina propria leukocytes).
Unstimulated cells that were otherwise treated identically served as a negative control.
Flow diagrams are shown gating on viable CD3+CD4+ T cells.
