Mouse ASCL1/Mash1 Antibody

Name: Mouse ASCL1/Mash1 Antibody
Brand: R&D Systems
SKU: AF2567
Rating: 4 (2 reviews)

Catalog #: AF2567
2 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2567
AF2567-SP

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Citations (2)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ASCL1/Mash1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse ASCL1/Mash1
His74-Asp173 (Gln160Glu)
Accession # Q53WV9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ASCL1/Mash1

Immunohistochemistry

3-15 µg/mL

Immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ASCL1/Mash1 in Mouse Embryo. ASCL1/Mash1 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS) using Goat Anti-Mouse ASCL1/Mash1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2567) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASCL1/Mash1

Mash1, also known as achaete-scute complex-like 1 (ASCL1), is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. The mouse immunogen used to generate this antibody shares 97% amino acid sequence identity with the equivalent region of human Mash1.

Long Name

Achaete Scute Complex-like 1

Entrez Gene IDs

429 (Human); 17172 (Mouse)

Alternate Names

achaete-scute complex (Drosophila) homolog-like 1; achaete-scute complex homolog 1 (Drosophila); achaete-scute complex-like 1 (Drosophila); achaete-scute homolog 1; ASCL1; ASH1; ASH-1; ASH1MASH1; BHLHA46; bHLHa46achaete-scute complex-like 1; Class A basic helix-loop-helix protein 46; HASH1; HASH1achaete scute protein; Mash1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse ASCL1/Mash1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The dual origin of the peripheral olfactory system: placode and neural crest.
Mol Brain, 2011-09-23;4(0):34.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Characterization of progenitor domains in the developing mouse thalamus.
Authors: Vue TY, Aaker J, Taniguchi A
J. Comp. Neurol., 2007-11-01;505(1):73-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr