Mouse BMP-15/GDF-9B Antibody

Catalog #: MAB5917 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5917
MAB5917-SP

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Mouse BMP-15/GDF-9B Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BMP‑15/GDF‑9B in direct ELISAs. In this format, no cross-reactivity with recombinant human BMP1.1, 2, 3, 3b, 4, 5, 6, 7, 8, 9, 10, 15, recombinant mouse BMP-3, 3b, 4, 5, 6, 7, 8b, 9, or 10 is observed.

Source

Monoclonal Rat IgG_2A Clone # 582703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP‑15/GDF‑9B
Gln268-Arg392
Accession # Q9Z0L4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BMP‑15/GDF‑9B in Mouse Ovary. BMP-15/GDF-9B was detected in perfusion fixed frozen sections of adult mouse ovary using Mouse BMP-15/GDF-9B Monoclonal Antibody (Catalog # MAB5917) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to an oocyte within the ovary. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a member of the TGF-beta superfamily. Mature BMP-15 has three intrachain disulfide bands that form a cysteine-knot fold. It is secreted as a 34 kDa non-disulfide-linked homodimer and as a 37 kDa nondisulfide heterodimer with GDF-9. BMP-15 is a product of oocytes and promotes granulosa cell proliferation and stem cell factor secretion. Mature mouse BMP-15 shares 44% aa sequence identity with mature mouse GDF-9. It also shares 70% and 78% aa sequence identity with human and sheep BMP-15, respectively.

Long Name

Bone Morphogenetic Protein 15

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Alternate Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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