Mouse BTN1A1/Butyrophilin Antibody

Catalog # Availability Size / Price Qty
AF4765
AF4765-SP
Detection of Mouse BTN1A1/Butyrophilin by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse BTN1A1/Butyrophilin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BTN1A1/Butyrophilin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human BTN1A1/Butyrophilin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BTN1A1/Butyrophilin
Ala27-Trp247
Accession # Q62556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse BTN1A1/Butyrophilin antibody by Western Blot. View Larger

Detection of Mouse BTN1A1/Butyrophilin by Western Blot. Western blot shows lysates of mouse lactating mammary tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse BTN1A1/Butyrophilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4765) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN1A1/Butyrophilin at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN1A1/Butyrophilin

Butyrophilin (Greek for “being like butter”; also Btn1a1) is a 66 kDa member of the BTN/MOG family of proteins. It is expressed on secretory mammary epithelium and associates with xanthine dehydrogenase. This is necessary for milk-lipid droplet secretion. Mature mouse Butyrophilin is 498 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 221 aa extracellular domain (ECD) (aa 27‑247) and a 256 aa cytoplasmic region. The ECD contains two V-type Ig‑like domains (aa 29‑141 and aa 149‑235). There are two potential splice variants. One shows a deletion of aa 145‑238, while a second shows a 36 aa substitution for aa 144‑524. Over aa 27‑247, mouse Butyrophilin shares 85% and 67% aa identity with rat and human Butyrophilin, respectively.

Entrez Gene IDs
696 (Human); 12231 (Mouse); 306956 (Rat)
Alternate Names
BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1); BTN; BTN1A1; butyrophilin subfamily 1 member A1; butyrophilin, subfamily 1, member A1

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse BTN1A1/Butyrophilin Antibody

There are currently no reviews for this product. Be the first to review Mouse BTN1A1/Butyrophilin Antibody and earn rewards!

Have you used Mouse BTN1A1/Butyrophilin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review