Mouse C-Reactive Protein/CRP ELISA Kit - Quantikine
Mouse C-Reactive Protein/CRP Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.453 | 1.31 | 3.78 | 0.48 | 1.33 | 3.76 |
Standard Deviation | 0.035 | 0.083 | 0.089 | 0.052 | 0.087 | 0.314 |
CV% | 7.7 | 6.3 | 2.4 | 10.8 | 6.5 | 8.4 |
Recovery
The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 95 | 82-115 |
Linearity
Scientific Data
Preparation and Storage
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice
Authors: P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan
BMC complementary medicine and therapies, 2022-08-04;22(1):208.
Species: Mouse
Sample Types: Serum
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Actions of Esomeprazole on the Maternal Vasculature in Lean and Obese Pregnant Mice with Impaired Nitric Oxide Synthesis: A Model of Preeclampsia
Authors: N de Alwis, NK Binder, YTM Mangwiro, S Beard, N Pritchard, E Kadife, BR Fato, E Keenan, FC Brownfoot, TJ Kaitu'u-Li, NJ Hannan
International Journal of Molecular Sciences, 2022-07-25;23(15):.
Species: Mouse
Sample Types: Serum
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Pirfenidone increases IL10 and improves acute pancreatitis in multiple clinically relevant murine models
Authors: E Palathinga, J George, M Tarique, S Iyer, P Sahay, B Gomez Agui, DB Edwards, B Giri, V Sethi, T Jain, P Sharma, U Vaish, HK C Jacob, A Ferrantell, CL Maynard, AK Saluja, RK Dawra, V Dudeja
JCI Insight, 2022-01-25;0(0):.
Species: Mouse
Sample Types: Serum
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Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
Aging Cell, 2021-10-23;0(0):e13501.
Species: Mouse
Sample Types: Serum
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Pleiotropic Effects of Atorvastatin Result in a Downregulation of the Carboxypeptidase U System (CPU, TAFIa, CPB2) in a Mouse Model of Advanced Atherosclerosis
Authors: K Claesen, L Roth, JC Mertens, K Hermans, Y Sim, D Hendriks
Pharmaceutics, 2021-10-19;13(10):.
Species: Mouse
Sample Types: Plasma
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Circulating Endothelial Progenitor Cells Are Preserved in Female Mice Exposed to Ambient Fine Particulate Matter Independent of Estrogen
Authors: X Liu, Y Xiao, Q Zhu, Y Cui, H Hao, M Wang, PJ Cowan, RJ Korthuis, G Li, Q Sun, Z Liu
International Journal of Molecular Sciences, 2021-07-04;22(13):.
Species: Mouse
Sample Types: Serum
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An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
Authors: JK Nam, AR Kim, SH Choi, JH Kim, KJ Choi, S Cho, JW Lee, HJ Cho, YW Kwon, J Cho, KS Kim, J Kim, HJ Lee, TS Lee, S Bae, HJ Hong, YJ Lee
Nature Communications, 2021-06-02;12(1):3279.
Species: Mouse
Sample Types: Serum
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Inflammation-driven senescence-associated secretory phenotype in cancer-associated fibroblasts enhances peritoneal dissemination
Authors: T Yasuda, M Koiwa, A Yonemura, K Miyake, R Kariya, S Kubota, T Yokomizo-N, N Yasuda-Yos, T Uchihara, R Itoyama, L Bu, L Fu, K Arima, D Izumi, S Iwagami, K Eto, M Iwatsuki, Y Baba, N Yoshida, H Ohguchi, S Okada, K Matsusaki, G Sashida, A Takahashi, P Tan, H Baba, T Ishimoto
Cell Reports, 2021-02-23;34(8):108779.
Species: Mouse
Sample Types: Serum
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Metformin treatment of high-fat diet-fed obese male mice restores sperm function and fetal growth, without requiring weight loss
Authors: NO McPherson, M Lane
Asian J. Androl., 2020-11-01;0(0):.
Species: Mouse
Sample Types: Plasma
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Lactobacillus fermentum promotes adipose tissue oxidative phosphorylation to protect against diet-induced obesity
Authors: Y Yoon, G Kim, MG Noh, JH Park, M Jang, S Fang, H Park
Exp. Mol. Med., 2020-09-11;0(0):.
Species: Mouse
Sample Types: Serum
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Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
Microorganisms, 2020-06-04;8(6):.
Species: Mouse
Sample Types: Serum
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Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet
Authors: J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob
J. Nutr. Biochem., 2020-06-02;84(0):108441.
Species: Mouse
Sample Types: Serum
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HIF1alpha inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis
Authors: C Liang, J Li, C Lu, D Xie, J Liu, C Zhong, X Wu, R Dai, H Zhang, D Guan, B Guo, B He, F Li, X He, W Zhang, BT Zhang, G Zhang, A Lu
Nat Commun, 2019-10-08;10(1):4579.
Species: Mouse
Sample Types: Serum
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REDD-1 aggravates endotoxin-induced inflammation via atypical NF-?B activation
Authors: DK Lee, JH Kim, J Kim, S Choi, M Park, W Park, S Kim, KS Lee, T Kim, J Jung, YK Choi, KS Ha, MH Won, TR Billiar, YG Kwon, YM Kim
FASEB J., 2018-03-16;0(0):fj201701436R.
Species: Mouse
Sample Types: Serum
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Berberine inhibits the ischemia-reperfusion injury induced inflammatory response and apoptosis of myocardial cells through the phosphoinositide 3-kinase/RAC-? serine/threonine-protein kinase and nuclear factor-?B signaling pathways
Authors: L Wang, H Ma, Y Xue, H Shi, T Ma, X Cui
Exp Ther Med, 2017-11-27;15(2):1225-1232.
Species: Mouse
Sample Types: Serum
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