Mouse Carbohydrate Sulfotransferase 4/CHST4 Antibody

Catalog #: AF5547 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5547
AF5547-SP

Detection of Mouse Carbohydrate Sulfotransferase 4/ CHST4 by Western Blot.

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All Carbohydrate Sulfotransferase 4/CHST4 Antibodies

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Mouse Carbohydrate Sulfotransferase 4/CHST4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Carbohydrate Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross‑reactivity with recombinant mouse CHST5 and rhCHST6 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4
Arg30-Gly388

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Neutralization

Measured by its ability to neutralize Recombinant Mouse Carbohydrate Sulfotransferase 4/CHST4 (8.33 µg/mL, Catalog # 5547-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to beta ‑D‑GlcNAc-1→6)-alpha -D-Man→OMe (XXX nM). The Neutralization Dose (ND₅₀) is typically 60 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Carbohydrate Sulfotransferase 4/ CHST4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5547) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 4/CHST4

The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of the recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). Human CHST4, also known as high endothelial cell N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferase (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycans (5). Mouse CHST4 shares 72% amino acid sequence identity with the human ortholog.

References

deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.
Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.
Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
MacRae, I.J. et al. (2000) Biochemistry. 39: 1613.
Wu, Z.L. et al. (2002) Faseb J. 16:539.

Entrez Gene IDs

10164 (Human); 26887 (Mouse); 307838 (Rat)

Alternate Names

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; Carbohydrate Sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.17; EC 2.8.2.21; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2