Home / Carbonic Anhydrase IV/CA4 / Mouse Carbonic Anhydrase IV/CA4 Antibody

Mouse Carbonic Anhydrase IV/CA4 Antibody

Catalog #: AF2414
19 Citations Datasheet / COA / SDS
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AF2414
AF2414-SP
Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot.
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Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Carbonic Anhydrase IV/CA4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human CA4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4
Glu18-Ser277
Accession # Q64444
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
12.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IV (Catalog # 2414-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Carbonic Anhydrase IV/CA4 antibody by Western Blot. View Larger

Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Carbonic Anhydrase IV/CA4 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Carbonic Anhydrase IV/CA4 in Mouse Kidney. Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Carbonic Anhydrase IV/CA4 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse Carbonic Anhydrase IV/CA4 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IV/CA4

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Tamai, S. et al. (1996) Biochem. Genet. 34:31.
Entrez Gene IDs
762 (Human); 12351 (Mouse)
Alternate Names
CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; Carbonic Anhydrase IV; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC 4.2.1.1; retinitis pigmentosa 17 (autosomal dominant); RP17

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Citations for Mouse Carbonic Anhydrase IV/CA4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Lung injury-induced activated endothelial cell states persist in aging-associated progressive fibrosis
    Authors: Raslan, AA;Pham, TX;Lee, J;Kontodimas, K;Tilston-Lunel, A;Schmottlach, J;Hong, J;Dinc, T;Bujor, AM;Caporarello, N;Thiriot, A;von Andrian, UH;Huang, SK;Nicosia, RF;Trojanowska, M;Varelas, X;Ligresti, G;
    Nature communications
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients
    Authors: McCauley HA, Riedman AM, Enriquez JR et al.
    Cellular and Molecular Gastroenterology and Hepatology
  3. Bisphosphonate affects the behavioral responses to HCl by disrupting farnesyl diphosphate synthase in mouse taste bud and tongue epithelial cells
    Authors: A Oike, S Iwata, A Hirayama, Y Ono, Y Nagasato, Y Kawabata, S Takai, K Sanematsu, N Wada, N Shigemura
    Scientific Reports, 2022-12-08;12(1):21246.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Carbonic anhydrase 4 disruption decreases synaptic and behavioral adaptations induced by cocaine withdrawal
    Authors: SC Gupta, A Ghobbeh, RJ Taugher-He, R Fan, JB Hardie, RT LaLumiere, JA Wemmie
    Science Advances, 2022-11-16;8(46):eabq5058.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Lipopolysaccharide-induced inflammation increases nitric oxide production in taste buds
    Authors: Z Wu, Y Huang, W Hu, L Ren, P Jiang, RF Margolskee, H Wang, S Feng
    Brain, Behavior, and Immunity, 2022-04-18;103(0):145-153.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Optogenetic Stimulation of Type I GAD65+ Cells in Taste Buds Activates Gustatory Neurons and Drives Appetitive Licking Behavior in Sodium-Depleted Mice
    Authors: C Baumer-Har, MA Raymond, TA Myers, KM Sussman, ST Rynberg, AP Ugartechea, D Lauterbach, TG Mast, JM Breza
    J. Neurosci., 2020-09-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Expression of protocadherin-20 in mouse taste buds
    Authors: F Hirose, S Takai, I Takahashi, N Shigemura
    Sci Rep, 2020-02-06;10(1):2051.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ISH-IHC
  8. Rewiring the taste system
    Authors: H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba
    Nature, 2017-08-09;548(7667):330-333.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
    Authors: W Ren, E Aihara, W Lei, N Gheewala, H Uchiyama, RF Margolskee, K Iwatsuki, P Jiang
    Sci Rep, 2017-06-21;7(1):4004.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. The cellular mechanism for water detection in the mammalian taste system
    Authors: D Zocchi, G Wennemuth, Y Oka
    Nat. Neurosci., 2017-05-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Bortezomib alters sour taste sensitivity in mice
    Authors: A Ohishi, K Nishida, K Miyamoto, M Imai, R Nakanishi, K Kobayashi, A Hayashi, K Nagasawa
    Toxicol Rep, 2017-03-10;4(0):172-180.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Bcl11b/Ctip2 is required for development of lingual papillae in mice
    Authors: Yugo Nishiguchi
    Dev Biol, 2016-06-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
    Authors: Wandernoth P, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker H, Sly W, Wennemuth G
    J Biol Chem, 2015-10-20;290(49):29202-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.
    Authors: Castillo D, Seidel K, Salcedo E, Ahn C, de Sauvage F, Klein O, Barlow L
    Development, 2014-07-03;141(15):2993-3002.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
    Authors: Wen T, Mingler M, Wahl B, Khorki M, Pabst O, Zimmermann N, Rothenberg M
    J Immunol, 2014-05-07;192(12):5481-9.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  16. Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
    Authors: Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A
    PLoS ONE, 2014-05-01;9(5):e96007.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
    Authors: Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
    PLoS ONE, 2012-04-19;7(4):e35588.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. CO2 chemosensing in rat oesophagus.
    Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
    Gut, 2008-08-05;57(12):1654-64.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  19. Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
    Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
    Gastroenterology, 2006-07-01;131(1):142-52.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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