Mouse Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Catalog #: MAB11231 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11231
MAB11231-SP

All Cathepsin A/Lysosomal Carboxypeptidase A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin A/Lysosomal Carboxypeptidase A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cathepsin A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse DPPIV/CD26 is observed.

Source

Monoclonal Rat IgG_2B Clone # 191424

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin A
aa 24-474

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse spinal cord

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin A/
Lysosomal Carboxypeptidase A, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1, 2). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal cathepsin A plays a role in regulation of functions of these molecules (3). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

References

Galjart, et al. (1990) J. Biol. Chem. 265:4678.
Pshezhetsky (1998) in Handbook of Proteolytic Enzymes (ed. Barrett, Rawlings, Woessner) pp. 393, Academic Press, San Diego.
Hiraiwa (1999) Cell. Mol. Life. Sci. 56:894.

Entrez Gene IDs

5476 (Human); 19025 (Mouse)

Alternate Names

beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; cathepsin ANGBE; CTSA; EC 3.4.16; EC 3.4.16.5; GLB2; GSL; Lysosomal Carboxypeptidase A; lysosomal protective protein; PPCA; PPGBprotective protein for beta-galactosidase (galactosialidosis); Protective protein cathepsin A; Protective protein for beta-galactosidase