Mouse CCL1/I-309/TCA-3 Antibody

Name: Mouse CCL1/I-309/TCA-3 Antibody
Brand: R&D Systems
SKU: MAB8451
Rating: 4 (1 reviews)

Catalog #: MAB8451
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8451
MAB8451-SP

Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL1/I-309/TCA-3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.

Source

Monoclonal Rat IgG_2B Clone # 148107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3 Lys24-Cys92 Accession # P10146

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Mouse CCL1/TCA-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF845)

Standard: Recombinant Mouse CCL1/TCA-3 Protein (Catalog # 845-TC)

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND₅₀) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody. Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/ TCA-3 Monoclonal Antibody (Catalog # MAB8451). The ND₅₀ is typically 0.6-3.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Mouse TCA-3 is a member of the CC beta family of chemokines, designated CCL1. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, is the orthologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. TCA-3 cDNA encodes a 92 aa precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

References

Goya, I. et al. (1998) J. Immunol. 160:1975.
Burd, P. et al. (1987) J. Immunol. 139:3126.

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Alternate Names

C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

Product Datasheets

Product Datasheet

COA

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Citations for Mouse CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
Am J Pathol, 2014-12-06;185(2):420-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Authors: Kearley J, Robinson DS, Lloyd CM
J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development