Home / CCL2/JE/MCP-1 / Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

Catalog #: BAF479
15 Citations Datasheet / COA / SDS
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BAF479
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Citations (15)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL2/JE/MCP‑1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)
Gln24-Arg96
Accession # Q5SVU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)

Mouse CCL2/JE/MCP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL2/JE/MCP‑1 Antibody (Catalog # AF-479-NA)

Standard: Recombinant Mouse CCL2/JE/MCP-1 Protein (Catalog # 479-JE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

References
  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.
Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Citations for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 2015-11-17;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  2. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
    Authors: Meng Y, Efimova EV, Hamzeh KW
    Mol. Ther., 2012-02-14;20(5):1046-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  4. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011-07-25;6(7):e22504.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  7. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  8. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006-07-01;177(1):612-20.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  9. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005-11-03;107(5):2112-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  11. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004-05-13;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
    Authors: Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A
    Immunol. Lett., 2002-06-03;82(1):111-21.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001-11-05;194(9):1375-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
    Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
    J. Immunol., 2000-03-01;164(5):2650-9.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development

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