Mouse CCL22/MDC Antibody

Name: Mouse CCL22/MDC Antibody
Brand: R&D Systems
SKU: MAB4391
Rating: 5 (1 reviews)

Catalog #: MAB4391
6 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4391-SP
MAB4391-100
MAB4391-500

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.

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Citations (6)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL22/MDC Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.

Source

Monoclonal Rat IgG_2A Clone # 158132

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse CCL22/MDC Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CCL22/MDC Biotinylated Antibody (Catalog # BAF439)

Standard: Recombinant Mouse CCL22/MDC Protein (Catalog # 439-MD)

Neutralization

Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL22/MDC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL22/MDC Monoclonal Antibody (Catalog # MAB4391). The ND₅₀ is typically 0.4-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL22/MDC

CCL22, also known as ABCD-1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

References

Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Alternate Names

A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CCL22/MDC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Ccl22 Diverts T Regulatory Cells and Controls the Growth of Melanoma
Authors: Jared Klarquist, Kristen Tobin, Peyman Farhangi Farhangi Oskuei, Steven W. Henning, Manuel F. Fernandez, Emilia R. Dellacecca et al.
Cancer Research
CCL22-specific Antibodies Reveal That Engagement of Two Distinct Binding Domains on CCL22 Is Required for CCR4-mediated Function
Authors: Sandra Santulli-Marotto, John Wheeler, Eilyn R. Lacy, Ken Boakye, Jennifer Luongo, Sheng-Jiun Wu et al.
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Authors: Kearley J, Robinson DS, Lloyd CM
J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development
iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
J. Immunol., 2007-10-01;179(7):4661-71.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization