Home / CCL28 / Mouse CCL28 Antibody

Mouse CCL28 Antibody

Catalog #: AF533
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF533
AF533-SP
Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL28 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL28 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CCL28 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL28 (Catalog # 533-VI)
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. View Larger

Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF533). The ND50 is typically 5-25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control.
    Authors: Cha HR, Ko HJ, Kim ED
    J. Immunol., 2011-08-10;187(6):3044-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009-06-01;182(11):6851-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
    Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
    J. Immunol., 2004-09-15;173(6):3668-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
    Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
    J. Immunol., 2003-02-01;170(3):1452-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CCL28 Antibody

There are currently no reviews for this product. Be the first to review Mouse CCL28 Antibody and earn rewards!

Have you used Mouse CCL28 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review