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Mouse CCL3/MIP-1 alpha Antibody

Catalog #: AF-450-NA
20 Citations 3 Reviews Datasheet / COA / SDS
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AF-450-NA
AF-450-SP
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Detection of Mouse CCL3/MIP‑1 alpha by Western Blot.
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Citations (20)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL3/MIP-1 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL3/MIP-1 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) CCL3, recombinant mouse (rm) CCL9/10, and rmCCL4 and less than 0.4% cross-reactivity with rhCCL7.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL3/MIP-1 alpha
Ala24-Ala92
Accession # Q5QNW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Mouse CCL3/MIP-1 alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL3/MIP‑1 alpha Biotinylated Antibody (Catalog # BAF450)

Standard: Recombinant Mouse CCL3/MIP-1 alpha Protein (Catalog # 450-MA)

Neutralization
Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL3/MIP‑1 alpha Isoform LD78a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CCL3/MIP-1a antibody by Western Blot. View Larger

Detection of Mouse CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry CCL3/MIP-1a antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

CCL3/MIP‑1 alpha in Mouse Thymus. CCL3/MIP-1a was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry CCL3/MIP-1a antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

CCL3/MIP‑1 alpha in RAW 264.7 Mouse Cell Line. CCL3/MIP-1a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/MIP‑1 alpha Antibody. View Larger

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/MIP‑1 alpha Antibody. Recombinant Mouse CCL3/MIP-1a (Catalog # 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/ MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA). The ND50 is typically 0.05-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

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Citations for Mouse CCL3/MIP-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Deficiency of lncRNA MERRICAL abrogates macrophage chemotaxis and diabetes-associated atherosclerosis
    Authors: Chen, J;Jamaiyar, A;Wu, W;Hu, Y;Zhuang, R;Sausen, G;Cheng, HS;de Oliveira Vaz, C;Pérez-Cremades, D;Tzani, A;McCoy, MG;Assa, C;Eley, S;Randhawa, V;Lee, K;Plutzky, J;Hamburg, NM;Sabatine, MS;Feinberg, MW;
    Cell reports
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity
    Authors: P Bhattachar, N Ismail, A Saxena, S Gannavaram, R Dey, T Oljuskin, A Akue, K Takeda, J Yu, S Karmakar, PK Dagur, JP McCoy, HL Nakhasi
    PloS Neglected Tropical Diseases, 2022-02-22;16(2):e0010224.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain
    Authors: AM Osman, Y Sun, TC Burns, L He, N Kee, N Oliva-Vila, A Alevyzaki, K Zhou, L Louhivuori, P Uhlén, E Hedlund, C Betsholtz, VM Lauschke, J Kele, K Blomgren
    Cell Rep, 2020-06-02;31(9):107699.
    Species: Mouse
    Sample Types:
    Applications: IHC
  4. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019-02-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation
    Authors: W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing
    Nat Commun, 2018-12-03;9(1):5127.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017-04-20;7(0):46514.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy
    Authors: David Blum
    Brain, 2016-11-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  9. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Recruitment and differentiation of conventional dendritic cell precursors in tumors.
    Authors: Diao J, Zhao J, Winter E, Cattral MS
    J. Immunol., 2009-12-21;184(3):1261-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
    Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
    J. Biol. Chem., 2009-07-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
    Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
    J. Immunol., 2008-11-01;181(9):6384-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008-08-11;205(9):2075-84.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Species: Mouse
    Sample Types: Tissue Secretion
    Applications: ELISA Development
  17. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  18. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005-02-02;128(0):854-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003-05-01;71(5):2634-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
    Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
    J. Immunol., 2001-08-01;167(3):1584-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development

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Reviews for Mouse CCL3/MIP-1 alpha Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Mouse CCL3/MIP-1 alpha Antibody
By Anonymous on 08/08/2019
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse CCL3/MIP-1 alpha Antibody AF-450-NA
Mouse CCL3/MIP-1 alpha Antibody
By José Luis Muñoz Madrigal on 03/27/2019
Application: WB Sample Tested: Brain (cerebral cortex) Species: Mouse
Mouse CCL3/MIP-1 alpha Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

This antibody was paired with BAF450 to build a sandwich ELISA to measure MIP-1a in mouse plasma.

ELISA Mouse CCL3/MIP-1 alpha Antibody AF-450-NA